TY  - JOUR
AU  - Blanchet, Clement
AU  - Round, Adam
AU  - Mertens, Haydyn D. T.
AU  - Ayyer, Kartik
AU  - Gräwert, Melissa Ann
AU  - Awel, Salah
AU  - Franke, Daniel
AU  - Dörner, Katerina
AU  - Bajt, Saša
AU  - Bean, Richard
AU  - Custódio, Tânia F.
AU  - de Wijn, Raphael
AU  - Juncheng, E.
AU  - Henkel, Alessandra
AU  - Gruzinov, Andrey
AU  - Jeffries, Cy M.
AU  - Kim, Yoonhee
AU  - Kirkwood, Henry
AU  - Kloos, Marco
AU  - Knoška, Juraj
AU  - Koliyadu, Jayanath
AU  - Letrun, Romain
AU  - Loew, Christian
AU  - Makroczyova, Jana
AU  - Mall, Abhishek
AU  - Meijers, Rob
AU  - Pena Murillo, Gisel Esperanza
AU  - Oberthür, Dominik
AU  - Round, Ekaterina
AU  - Seuring, Carolin
AU  - Sikorski, Marcin
AU  - Vagovic, Patrik
AU  - Valerio, Joana
AU  - Wollweber, Tamme
AU  - Zhuang, Yulong
AU  - Schulz, Joachim
AU  - Haas, Heinrich
AU  - Chapman, Henry N.
AU  - Mancuso, Adrian P.
AU  - Svergun, Dmitri
TI  - Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS)
JO  - Communications biology
VL  - 6
IS  - 1
SN  - 2399-3642
CY  - London
PB  - Springer Nature
M1  - PUBDB-2023-06274
SP  - 1057
PY  - 2023
AB  - Free-electron lasers (FEL) are revolutionizing X-ray-based structural biology methods. While protein crystallography is already routinely performed at FELs, Small Angle X-ray Scattering (SAXS) studies of biological macromolecules are not as prevalent. SAXS allows the study of the shape and overall structure of proteins and nucleic acids in solution, in a quasi-native environment. In solution, chemical and biophysical parameters that have an influence on the structure and dynamics of molecules can be varied and their effect on conformational changes can be monitored in time-resolved XFEL and SAXS experiments. We report here the collection of scattering form factors of proteins in solution using FEL X-rays. The form factors correspond to the scattering signal of the protein ensemble alone; the scattering contributions from the solvent and the instrument are separately measured and accurately subtracted. The experiment was done using a liquid jet for sample delivery. These results pave the way for time-resolved studies and measurements from dilute samples, capitalizing on the intense and short FEL X-ray pulses.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:001115957900003
DO  - DOI:10.1038/s42003-023-05416-7
UR  - https://bib-pubdb1.desy.de/record/596784
ER  -