Contribution to a book

PUBDB-2023-00142

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Mix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates

Ding, J. (Editor) ; Stagno, J. R. (Editor)Extern*XFEL.EU* ; Wang, Y.-X. (Editor)Extern* ; Stagno, J. R. (Corresponding author)Extern*XFEL.EU* ; Knoska, J.CFEL*DESY* ; Chapman, H. N.CFEL*XFEL.EU*DESY* ; Wang, Y.-X. (Corresponding author)Extern*

2023
Springer US New York, NY
ISBN: 978-1-0716-2687-0, 978-1-0716-2687-0 (electronic)

This record in other databases:  

Please use a persistent id in citations: doi:10.1007/978-1-0716-2687-0_16

Abstract: Time-resolved structure determination of macromolecular conformations and ligand-bound intermediates is extremely challenging, particularly for RNA. With rapid technological advances in both microfluidic liquid injection and X-ray free electron lasers (XFEL), a new frontier has emerged in time-resolved crystallography whereby crystals can be mixed with ligand and then probed with X-rays (mix-and-inject) in real time and at room temperature. This chapter outlines the basic setup and procedures for mix-and-inject experiments for recording time-resolved crystallographic data of riboswitch RNA reaction states using serial femtosecond crystallography (SFX) and an XFEL.

Keyword(s): Genetics (LCSH) ; Materials—Microscopy (LCSH)

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
3. Leibniz Preis - Leibiz Programm 2015: Prof. Dr. Henry N. Chapman (DFG-Leibniz-2015-Chapman) (DFG-Leibniz-2015-Chapman)

Experiment(s):

1. Experiments at CFEL

Appears in the scientific report 2023

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Database coverage:
; SCOPUS

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