%0 Book Section
%A Stagno, Jason R.
%A Knoska, Juraj
%A Chapman, Henry N.
%A Wang, Yun-Xing
%Y Ding, Jienyu
%Y Stagno, Jason R.
%Y Wang, Yun-Xing
%T Mix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates; 1st ed. 2023
%V 2568
%C New York, NY
%I Springer US
%M PUBDB-2023-00142
%@ 978-1-0716-2687-0
%B Methods in Molecular Biology
%P 243-249
%D 2023
%< RNA Structure and Dynamics / Ding, Jienyu (Editor) ; New York, NY : Springer US, 2023, Chapter 16 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-2686-3=978-1-0716-2687-0 ; doi:10.1007/978-1-0716-2687-0
%X Time-resolved structure determination of macromolecular conformations and ligand-bound intermediates is extremely challenging, particularly for RNA. With rapid technological advances in both microfluidic liquid injection and X-ray free electron lasers (XFEL), a new frontier has emerged in time-resolved crystallography whereby crystals can be mixed with ligand and then probed with X-rays (mix-and-inject) in real time and at room temperature. This chapter outlines the basic setup and procedures for mix-and-inject experiments for recording time-resolved crystallographic data of riboswitch RNA reaction states using serial femtosecond crystallography (SFX) and an XFEL. 
%F PUB:(DE-HGF)7
%9 Contribution to a book
%R 10.1007/978-1-0716-2687-0_16
%U https://bib-pubdb1.desy.de/record/491433