TY  - CHAP
AU  - Stagno, Jason R.
AU  - Knoska, Juraj
AU  - Chapman, Henry N.
AU  - Wang, Yun-Xing
A3  - Ding, Jienyu
A3  - Stagno, Jason R.
A3  - Wang, Yun-Xing
TI  - Mix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates; 1st ed. 2023
VL  - 2568
CY  - New York, NY
PB  - Springer US
M1  - PUBDB-2023-00142
SN  - 978-1-0716-2687-0
T2  - Methods in Molecular Biology
SP  - 243-249
PY  - 2023
AB  - Time-resolved structure determination of macromolecular conformations and ligand-bound intermediates is extremely challenging, particularly for RNA. With rapid technological advances in both microfluidic liquid injection and X-ray free electron lasers (XFEL), a new frontier has emerged in time-resolved crystallography whereby crystals can be mixed with ligand and then probed with X-rays (mix-and-inject) in real time and at room temperature. This chapter outlines the basic setup and procedures for mix-and-inject experiments for recording time-resolved crystallographic data of riboswitch RNA reaction states using serial femtosecond crystallography (SFX) and an XFEL. 
LB  - PUB:(DE-HGF)7
DO  - DOI:10.1007/978-1-0716-2687-0_16
UR  - https://bib-pubdb1.desy.de/record/491433
ER  -