Contribution to a book

Smart systems and data-driven services have the potential to answer medical needs in nanomedicine to get faster to the clinic and manufacturing stage. As an example from galenics, we explored the molecular packing within nanoparticles composed of fullerene C60 (C60) and the anthracycline antibiotic doxorubicin (Dox); the nanoparticle was previously proven to be a promising candidate for photodynamic and chemotherapeutic treatment of cancer [...]

The manufacturing and assembly of the CTAO Medium-Sized Telescopes (MST) is a complex process thatinvolves a geographically dispersed engineering collaboration. This collaboration must integrate all stakeholders,provide effective decision-making procedures, and establish reliable and efficient material and information flow.One of the key challenges in mastering this process is the digitalisation of manufacturing planning, coordinationand progress tracking processes based on a Product Lifecycle Management (PLM) system. [...]

The comprehension of microhole formation during percussion drilling of metals using ultrashort laser pulses is still limited. The shape of a microhole can be impacted by factors like heat accumulation, the emergence of side channels, bending, and bulging. [...]

The SASE3 Variable Polarization project is intended to offer polarization control of the X-ray FEL pulses at the European XFEL. The project was completed in early 2022. [...]

Membrane proteins are essential components of biological membranes with key roles in cellular processes such as nutrient transport, cell communication, signaling, or energy conversion. Due to their crucial functions, membrane proteins and their complexes are often targets for therapeutic interventions [...]

An Introduction to Rift Valley Fever Virus -- Aedes aegypti Vector Competence Assay for Rift Valley Fever Virus using Artificial Blood Meal -- Isolation of Rift Valley Fever Virus from Field Specimens using Cell Culture Approaches -- Detection and Diagnosis of Rift Valley Fever Virus -- Analysis of Negative-Strand RNA Viruses by RT-qPCR: Rift Valley Fever Virus and Toscana Virus -- Validated Methods for Effective Decontamination and Inactivation of Rift Valley Fever Virus -- Guidelines for In Vitro Production and Quantification of Rift Valley Fever Virus -- Cell-Free Synthesis of Bunyavirales Proteins in View of Their Structural Characterization by Nuclear Magnetic Resonance -- Expression and Purification of RVFV Glycoprotein C for Structural Studies using Baculovirus Expression System -- Next-Generation Sequencing and Proteomics-Enabled Approach for Rapid and High-Throughput Isolation of Virus-Neutralizing Nanobodies -- Use of Single-Domain Antibodies Against the NSm Protein for the Detection of Cells Infected by Rift Valley Fever Virus -- Making Rift Valley Fever Viral Particles Fluorescent -- Identification of RVFV Host Factors using Quantitative Interaction Proteomics -- Genome-Wide CRISPR-Cas9 Screening for the Identification of Host Dependency Factors of Emerging Viruses -- Preparation of Bunyavirus-Infected Cells for Electron Cryo-Tomography -- Imaging Bunyavirus Infections by Transmission Electron Microscopy: Conventional Sample Preparation vs High-Pressure Freezing and Freeze-Substitution -- The Phlebovirus Ribonucleoprotein: An Overview -- Nucleotide Resolution Mapping of Rift Valley Fever Virus Nucleoprotein - Genome RNA Interactions -- Characterization of RVFV Nucleocapsid Protein Binding Sites on RNA by iCLIP-seq -- Yeast Expression and Activity Assay of RVFV Polymerase -- Single-Molecule Visualization of Bunyavirus Genome Segments using Fluorescence In Situ Hybridization -- Single-Cell RNA-Sequencing of RVFV Infection -- Using RNAseq to Uncover Transcriptional and Splicing Differences in Host Cells during Rift Valley Fever Virus Infection -- Quantification of RVFV Specific T Cell Responses in Mice Pre-Immunized with Potential Vaccine Candidates -- Use of Human Macrophages to Study Bunyavirus NSs Functions -- 3D Spheroid and Organoid Models to Study Neuroinfection of RNA Viruses -- Laboratory Animal Models for Rift Valley Fever Virus Disease -- Monitoring RVFV Infection using Bioluminescent Reporter Viruses In Vivo

Challenges for Vaccinologists in the First Half of the Twenty First Century -- Principles in Immunology for the Design and Development of Vaccines -- Revisiting the Principles of Designing a Vaccine -- Status of COVID-19 Pandemic before the Administration of Vaccine -- mRNA Vaccines to Protect against Diseases -- Artificial Intelligence in Vaccine and Drug Design -- Vaccines Targeting Numerous Coronavirus Antigens, Ensuring Broader Global Population Coverage: Multi-Epitope and Multi-Patch Vaccines.-Use of Micro-computed Tomography to Visualize and Quantify COVID-19 Vaccine Efficiency in Free-breathing Hamsters -- Design of Replication-Competent VSV- and Ervebo-vectored Vaccines against SARS-CoV-2 -- CRISPR Engineering of Bacteriophage T4 to Design Vaccines against SARS-CoV-2 and Emerging Pathogens -- Techniques for Developing and Assessing Immune Responses Induced by Synthetic DNA Vaccines for Emerging Infectious Diseases -- Towards Determining the Epitopes of the Structural proteins of SARS-CoV-2 -- Development, production and characterization of hepatitis B subviral envelope particles as a third-generation vaccine -- Generation of CpG-Recoded Zika Virus Vaccine Candidates -- Salmonella Uptake into Gut-Associated Lymphoid Tissues: Implications for Targeted Mucosal Vaccine Design and Delivery -- Development of Human Recombinant Leptospirosis Vaccines -- Induction of T-cell Responses by Vaccination of a Streptococcus pneumoniae Whole Cell Vaccine -- Development of a Bacterial Nanoparticle Vaccine against Escherichia coli -- Construction of Novel Live Genetically Modified BCG Vaccine Candidates using Recombineering Tools -- An Update on Tuberculosis Vaccines -- Structure-Based Design of Diagnostics and Vaccines for Lyme Disease -- Development of a SONIX vaccine to protect against ehrlichiosis -- Development of the Anti-Leishmanial Vaccine -- In Silico Design of Recombinant Chimera T Cell Peptide Epitope Vaccines for Visceral Leishmaniasis -- Pre-clinical Assessment of the Immunogenicity of Experimental Leishmania Vaccines -- Production of Oral Vaccines Based on Retrovirus Virus-like Particles Pseudotyped with Protozoan-Surface Proteins -- A Fast-Track Phenotypic Characterization of Plasmodium falciparum Vaccine Antigens through Lyse-Reseal Erythrocytes Mediated Delivery (LyRED) of RNA Interference for Targeted Translational Repression -- Plasmodium falciparum Antigen Expression in Leishmania Parasite: A Way Forward for Live Attenuated Vaccine Development -- Molecular Characterization of a Vector-based Candidate Antigen using the 3'–RACE and Genome Walking Methods and In Silico Analysis of the Correspondent Protein for Vaccine Design and Development -- In vitro culture of Plasmodium falciparum for the Production of Mature Gametocytes for Performing Standard Membrane Feeding Assay and Infection of Anopheles spp. -- Plasmodium berghei Infection in BALB/c Mice Model as an Animal Model for Malaria Disease Research -- Standard membrane feeding assay for malaria transmission studies -- Generation of Tumor Targeted Dendritic Cell Vaccines with Improved Immunogenic and Migratory Phenotype -- Monocytes as a Cellular Vaccine Platform to Induce Anti-Tumor Immunity -- Beyond Sequencing: Prioritizing and Delivering Neoantigens for Cancer Vaccines -- Proteomics for Development of Food Allergy Vaccines -- Estimating Vaccine Potency using Antibody-based Competition Assays..

Identification and enumeration of Plasmodium falciparum parasites by light microscopy -- Collection and cryopreservation of Plasmodium falciparum clinical isolates in the field -- Preservation of parasite RNA in the field -- Preservation and extraction of malaria parasite DNA from dried blood spots -- Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum -- Genotyping of Plasmodium falciparum isolates to assess clone composition in parasite cultures -- Immunomagnetic selection of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants -- Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants -- Selecting Plasmodium falciparum infected erythrocytes for adhesion to cell lines -- Synchronisation of Plasmodium falciparum and P. knowlesi in vitro cultures using a highly specific protein kinase inhibitor -- Generation of Plasmodium falciparum gametocytes in vitro with specific considerations for field isolates -- Imaging of extracellular vesicles derived from Plasmodium falciparum-infected red blood cells using atomic force microscopy -- Analysis of var gene transcript patterns by quantitative real-time PCR -- Analysis of var gene transcription pattern using DBLα-tags -- RNAseq of infected erythrocyte surface antigen-encoding genes -- Resetting var gene transcription in Plasmodium falciparum -- CRISPR/Cas9 editing of the Plasmodium falciparum genome -- CRISPR-Cas9 editing of the Plasmodium falciparum genome - Special applications -- Extraction and immunoprecipitation of VAR2CSA, the PfEMP1 associated with placental malaria -- Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system -- Expression of large full-length PfEMP1 proteins in HEK293 cells -- Receptor affinity-based purification of PfEMP1 proteins -- Analysis of antibody reactivity to malaria antigens by microsphere-based multiplex immunoassay -- High-throughput BioPlex assay for the study of functionally active Plasmodium falciparum antigens that are expressed on the surface of infected erythrocytes -- Protein microarrays as a tool to analyze antibody responses to variant surface antigens expressed on the surface of Plasmodium falciparum-infected erythrocytes -- Protocol for differential biopanning of Plasmodium falciparum phage display cDNA library to identify parasite targets of protective antibodies -- Affinity purification of PfEMP1-specific antibodies from human blood -- Production of anti-PfEMP1 polyclonal antisera in rats and mice -- Production of PfEMP1-specific mouse monoclonal antibodie -- Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals -- Visualization of infected red blood cell surface antigens by fluorescence microscop -- Analysis by flow cytometry of α2‑macroglobulin and non-immune IgM-binding to Plasmodium falciparum-infected erythrocytes -- An improved method for assessing antigen presentation on the surface of Plasmodium falciparum-infected erythrocytes by immuno-electron microscopy -- Assessing antigen presentation on the surface of Plasmodium falciparum-infected erythrocytes by photoactivated localization microscopy (PALM) -- Surface plasmon resonance analysis of PfEMP1 interaction with receptors -- Analysis of antibody neutralization of PfEMP1 binding by competition ELISA -- Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay -- Bridging and clumping: investigating platelet interactions with Plasmodium falciparum-infected red blood cells and endothelial cells in cerebral malaria -- Assay of static adhesion of Plasmodium falciparum-infected erythrocytes to cells, including inhibition of the adhesion -- Static adhesion of Plasmodium falciparum-infected erythrocytes to purified and recombinant receptors -- Selecting Plasmodium falciparum-infected erythrocytes for adhesion to recombinant receptors under flow conditions -- Chip-based assay of adhesion of Plasmodium falciparum-infected erythrocytes to cells under flow -- Binding of Plasmodium falciparum-infected red blood cells to engineered 3D microvessels -- 3D organoid assay of the impact of Plasmodium falciparum-infected erythrocyte adhesion on the blood-brain barrier -- Receptor-functionalized lipid membranes as biomimetic surfaces for adhesion of Plasmodium falciparum-infected erythrocytes -- Antibody-dependent THP-1 cell-mediated phagocytosis of Plasmodium falciparum-infected erythrocytes -- Assaying interactions between neutrophils and Plasmodium falciparum-infected red blood cells -- Natural killer cell antibody dependent cellular cytotoxicity (ADCC) activity against Plasmodium falciparum-infected red blood cells -- Assessing PfGARP-mediated apoptosis of blood-stage Plasmodium falciparum parasites -- Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies -- Flow cytometry assay of Plasmodium falciparum-specific B-cell proportions -- Assessment of Plasmodium falciparum antigen-specific B cells -- Whole blood dendritic cell cytokine production assay -- Measuring the impact of malaria on the living human retina -- Ocular pathology of cerebral malaria -- Investigating interactions between endothelial cells and parasitised red blood cells in skin and subcutaneous tissue..

We present an update of the PhLAM-DESY project aiming at developing novel electro-optic diagnostics destined to record electron bunch shapes (and THz pulses in general). When high repetition rate operation is needed, a very efficient technique consists of probing the electric waveform of interest in an electro-optically active crystal using chirped laser probes. [...]

Linear accelerators for FELs have very high requirements for the accuracy of synchronization. The long and short term stability is influenced by various sources of interference. [...]