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000491433 041__ $$aEnglish
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000491433 1001_ $$0P:(DE-HGF)0$$aDing, Jienyu$$b0$$eEditor
000491433 245__ $$aMix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates
000491433 250__ $$a1st ed. 2023
000491433 260__ $$aNew York, NY$$bSpringer US$$c2023
000491433 29510 $$aRNA Structure and Dynamics / Ding, Jienyu (Editor) ; New York, NY : Springer US, 2023, Chapter 16 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-2686-3=978-1-0716-2687-0 ; doi:10.1007/978-1-0716-2687-0
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000491433 4900_ $$aMethods in Molecular Biology$$v2568
000491433 520__ $$aTime-resolved structure determination of macromolecular conformations and ligand-bound intermediates is extremely challenging, particularly for RNA. With rapid technological advances in both microfluidic liquid injection and X-ray free electron lasers (XFEL), a new frontier has emerged in time-resolved crystallography whereby crystals can be mixed with ligand and then probed with X-rays (mix-and-inject) in real time and at room temperature. This chapter outlines the basic setup and procedures for mix-and-inject experiments for recording time-resolved crystallographic data of riboswitch RNA reaction states using serial femtosecond crystallography (SFX) and an XFEL. 
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000491433 650_0 $$aGenetics
000491433 650_0 $$aMaterials—Microscopy
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000491433 7001_ $$0P:(DE-H253)PIP1081444$$aStagno, Jason R.$$b1$$eEditor
000491433 7001_ $$0P:(DE-H253)PIP1033497$$aWang, Yun-Xing$$b2$$eEditor
000491433 7001_ $$0P:(DE-H253)PIP1081444$$aStagno, Jason R.$$b3$$eCorresponding author
000491433 7001_ $$0P:(DE-H253)PIP1020244$$aKnoska, Juraj$$b4
000491433 7001_ $$0P:(DE-H253)PIP1006324$$aChapman, Henry N.$$b5
000491433 7001_ $$0P:(DE-H253)PIP1033497$$aWang, Yun-Xing$$b6$$eCorresponding author
000491433 773__ $$a10.1007/978-1-0716-2687-0_16
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