Journal Article

PUBDB-2020-00822

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Serial protein crystallography in an electron microscope

Bücker, R. ; Hogan-Lamarre, P.CFEL*MPG* ; Mehrabi, P. ; Schulz, E. C. ; Bultema, L. A.CFEL*EMBL* ; Gevorkov, Y.CFEL* ; Brehm, W.CFEL*DESY* ; Yefanov, O.CFEL*EMBL*DESY* ; Oberthür, D.CFEL*DESY* ; Kassier, G. H.CFEL*MPG* ; Miller, D. (Corresponding author)CFEL*MPG*

2020
Nature Publishing Group UK [London]

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Please use a persistent id in citations: doi:10.1038/s41467-020-14793-0  doi:10.3204/PUBDB-2020-00822

Abstract: Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures from sub-micron-sized crystals. However, beam time at these facilities is scarce, and involved sample delivery techniques are required. On the other hand, rotation electron diffraction (MicroED) has shown great potential as an alternative means for protein nano-crystallography. Here, we present a method for serial electron diffraction of protein nanocrystals combining the benefits of both approaches. In a scanning transmission electron microscope, crystals randomly dispersed on a sample grid are automatically mapped, and a diffraction pattern at fixed orientation is recorded from each at a high acquisition rate. Dose fractionation ensures minimal radiation damage effects. We demonstrate the method by solving the structure of granulovirus occlusion bodies and lysozyme to resolutions of 1.55 Å and 1.80 Å, respectively. Our method promises to provide rapid structure determination for many classes of materials with minimal sample consumption, using readily available instrumentation.

Note: open access

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Contributing Institute(s):

1. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
2. Forschungsgruppe für strukturelle Dynamik (MPSD)
3. MPSD (CFEL-ARD)
4. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. AXSIS - Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (609920) (609920)
3. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
4. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)

Experiment(s):

1. Experiments at CFEL

Appears in the scientific report 2020

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