TY  - JOUR
AU  - Bücker, Robert
AU  - Hogan-Lamarre, Pascal
AU  - Mehrabi, Pedram
AU  - Schulz, Eike C.
AU  - Bultema, Lindsey A.
AU  - Gevorkov, Yaroslav
AU  - Brehm, Wolfgang
AU  - Yefanov, Oleksandr
AU  - Oberthür, Dominik
AU  - Kassier, Günther H.
AU  - Miller, Dwayne
TI  - Serial protein crystallography in an electron microscope
JO  - Nature Communications
VL  - 11
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - PUBDB-2020-00822
SP  - 996
PY  - 2020
N1  - open access
AB  - Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures from sub-micron-sized crystals. However, beam time at these facilities is scarce, and involved sample delivery techniques are required. On the other hand, rotation electron diffraction (MicroED) has shown great potential as an alternative means for protein nano-crystallography. Here, we present a method for serial electron diffraction of protein nanocrystals combining the benefits of both approaches. In a scanning transmission electron microscope, crystals randomly dispersed on a sample grid are automatically mapped, and a diffraction pattern at fixed orientation is recorded from each at a high acquisition rate. Dose fractionation ensures minimal radiation damage effects. We demonstrate the method by solving the structure of granulovirus occlusion bodies and lysozyme to resolutions of 1.55 Å and 1.80 Å, respectively. Our method promises to provide rapid structure determination for many classes of materials with minimal sample consumption, using readily available instrumentation.
LB  - PUB:(DE-HGF)16
C6  - pmid:32081905
UR  - <Go to ISI:>//WOS:000517991000002
DO  - DOI:10.1038/s41467-020-14793-0
UR  - https://bib-pubdb1.desy.de/record/435853
ER  -