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000435853 1001_ $$00000-0001-7654-4707$$aBücker, Robert$$b0
000435853 245__ $$aSerial protein crystallography in an electron microscope
000435853 260__ $$a[London]$$bNature Publishing Group UK$$c2020
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000435853 520__ $$aSerial X-ray crystallography at free-electron lasers allows to solve biomolecular structures from sub-micron-sized crystals. However, beam time at these facilities is scarce, and involved sample delivery techniques are required. On the other hand, rotation electron diffraction (MicroED) has shown great potential as an alternative means for protein nano-crystallography. Here, we present a method for serial electron diffraction of protein nanocrystals combining the benefits of both approaches. In a scanning transmission electron microscope, crystals randomly dispersed on a sample grid are automatically mapped, and a diffraction pattern at fixed orientation is recorded from each at a high acquisition rate. Dose fractionation ensures minimal radiation damage effects. We demonstrate the method by solving the structure of granulovirus occlusion bodies and lysozyme to resolutions of 1.55 Å and 1.80 Å, respectively. Our method promises to provide rapid structure determination for many classes of materials with minimal sample consumption, using readily available instrumentation.
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000435853 7001_ $$0P:(DE-H253)PIP1033057$$aHogan-Lamarre, Pascal$$b1
000435853 7001_ $$00000-0003-3211-6959$$aMehrabi, Pedram$$b2
000435853 7001_ $$00000-0002-1685-8605$$aSchulz, Eike C.$$b3
000435853 7001_ $$0P:(DE-H253)PIP1080911$$aBultema, Lindsey A.$$b4
000435853 7001_ $$00000-0001-9273-7648$$aGevorkov, Yaroslav$$b5
000435853 7001_ $$0P:(DE-H253)PIP1033390$$aBrehm, Wolfgang$$b6
000435853 7001_ $$0P:(DE-H253)PIP1006155$$aYefanov, Oleksandr$$b7
000435853 7001_ $$0P:(DE-H253)PIP1012087$$aOberthür, Dominik$$b8
000435853 7001_ $$0P:(DE-H253)PIP1016255$$aKassier, Günther H.$$b9
000435853 7001_ $$0P:(DE-H253)PIP1012317$$aMiller, Dwayne$$b10$$eCorresponding author
000435853 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-020-14793-0$$gVol. 11, no. 1, p. 996$$n1$$p996$$tNature Communications$$v11$$x2041-1723$$y2020
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