Home > Publications database > The hit-and-return system enables efficient time-resolved serial synchrotron crystallography |
Journal Article | PUBDB-2019-00026 |
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2018
Nature Publishing Group
London [u.a.]
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Please use a persistent id in citations: doi:10.1038/s41592-018-0180-2 doi:10.3204/PUBDB-2019-00026
Abstract: We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
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