Journal Article PUBDB-2019-00026

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The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

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2018
Nature Publishing Group London [u.a.]

Nature methods 15(11), 901 - 904 () [10.1038/s41592-018-0180-2]
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Abstract: We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

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Note: © The Author(s), under exclusive licence to Springer Nature America, Inc.

Contributing Institute(s):
  1. DOOR-User (DOOR ; HAS-User)
  2. MPSD (CFEL-ARD)
  3. MPSD (CFEL-SSUMP)
Research Program(s):
  1. 6G3 - PETRA III (POF3-622) (POF3-622)
Experiment(s):
  1. PETRA Beamline P11 (PETRA III)
  2. PETRA Beamline P14 (PETRA III)

Appears in the scientific report 2018
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 Record created 2019-01-03, last modified 2025-07-29


Published on 2018-10-30. Available in OpenAccess from 2019-04-30.:
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