TY  - JOUR
AU  - Schulz, Eike C.
AU  - Mehrabi, Pedram
AU  - Müller-Werkmeister, Henrike M.
AU  - Tellkamp, Friedjof
AU  - Jha, Ajay
AU  - Stuart, William
AU  - Persch, Elke
AU  - De Gasparo, Raoul
AU  - Diederich, François
AU  - Pai, Emil F.
AU  - Miller, R. J. Dwayne
TI  - The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
JO  - Nature methods
VL  - 15
IS  - 11
SN  - 1548-7105
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - PUBDB-2019-00026
SP  - 901 - 904
PY  - 2018
N1  - © The Author(s), under exclusive licence to Springer Nature America, Inc.
AB  - We present a ‘hit-and-return’ (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.
LB  - PUB:(DE-HGF)16
C6  - pmid:30377366
UR  - <Go to ISI:>//WOS:000448827300022
DO  - DOI:10.1038/s41592-018-0180-2
UR  - https://bib-pubdb1.desy.de/record/417850
ER  -