Journal Article

PUBDB-2018-01143

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Supersaturation-controlled microcrystallization and visualization analysis for serial femtosecond crystallography

Lee, D. B. ; Kim, J.-M. ; Seok, J. H. ; Lee, J.-H. ; Jo, J. D. ; Mun, J. Y. ; Conrad, C.Extern* ; Coe, J. ; Nelson, G. ; Hogue, B.Extern* ; White, T. A.CFEL*DESY* ; Zatsepin, N.Extern* ; Weierstall, U.Extern* ; Barty, A.CFEL*DESY* ; Chapman, H.CFEL*DESY* ; Fromme, P.Extern* ; Spence, J.Extern* ; Chung, M. S. ; Oh, C.-H. (Corresponding author) ; Kim, K. H. (Corresponding author)

2018
Nature Publishing Group London

This record in other databases:        

Please use a persistent id in citations: doi:10.1038/s41598-018-20899-9  doi:10.3204/PUBDB-2018-01143

Abstract: Time-resolved serial femtosecond crystallography with X-ray free electron laser (XFEL) holds the potential to view fast reactions occurring at near-physiological temperature. However, production and characterization of homogeneous micron-sized protein crystals at high density remain a bottleneck, due to the lack of the necessary equipments in ordinary laboratories. We describe here supersaturation-controlled microcrystallization and visualization and analysis tools that can be easily used in any laboratory. The microcrystallization conditions of the influenza virus hemagglutinin were initially obtained with low reproducibility, which was improved by employing a rapid evaporation of hanging drops. Supersaturation-controlled microcrystallization was then developed in a vapor diffusion mode, where supersaturation was induced by evaporation in hanging drops sequentially for durations ranging from 30 sec to 3 min, depending on the protein. It was applied successfully to the microcrystal formation of lysozyme, ferritin and hemagglutinin with high density. Moreover, visualization and analysis tools were developed to characterize the microcrystals observed by light microscopy. The size and density distributions of microcrystals analyzed by the tools were found to be consistent with the results of manual analysis, further validated by high-resolution microscopic analyses. Our supersaturation-controlled microcrystallization and visualization and analysis tools will provide universal access to successful XFEL studies.

---

Contributing Institute(s):

1. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
2. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)

Experiment(s):

1. Experiments at CFEL
2. Measurement at external facility

Appears in the scientific report 2018

---

Database coverage:
; ; ; ; BIOSIS Previews ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

---

---

Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article Lee, D. B. ; Kim, J.-M. ; Seok, J. H. ; Lee, J.-H. ; Jo, J. D. ; Mun, J. Y. ; Conrad, C. ; Coe, J. ; Nelson, G. ; Hogue, B. ; White, T. A.CFEL*DESY* ; Zatsepin, N. ; Weierstall, U. ; Barty, A.CFEL*DESY* ; Chapman, H.CFEL*DESY* ; Fromme, P. ; Spence, J. ; Chung, M. S. ; Oh, C.-H. (Corresponding author) ; Kim, K. H. (Corresponding author)
Author Correction: Supersaturation-controlled microcrystallization and visualization analysis for serial femtosecond crystallography
Scientific reports 8(1), 6346 (2018) [10.1038/s41598-018-24178-5]2018     Files BibTeX | EndNote: XML, Text | RIS

---