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@ARTICLE{Lee:417951,
author = {Lee, Dan Bi and Kim, Jong-Min and Seok, Jong Hyeon and Lee,
Ji-Hye and Jo, Jae Deok and Mun, Ji Young and Conrad,
Chelsie and Coe, Jesse and Nelson, Garrett and Hogue, Brenda
and White, Thomas A. and Zatsepin, Nadia and Weierstall, Uwe
and Barty, Anton and Chapman, Henry and Fromme, Petra and
Spence, John and Chung, Mi Sook and Oh, Chang-Hyun and Kim,
Kyung Hyun},
title = {{A}uthor {C}orrection: {S}upersaturation-controlled
microcrystallization and visualization analysis for serial
femtosecond crystallography},
journal = {Scientific reports},
volume = {8},
number = {1},
issn = {2045-2322},
address = {London},
publisher = {Springer Nature},
reportid = {PUBDB-2019-00079},
pages = {6346},
year = {2018},
abstract = {Time-resolved serial femtosecond crystallography with X-ray
free electron laser (XFEL) holds the potential to view fast
reactions occurring at near-physiological temperature.
However, production and characterization of homogeneous
micron-sized protein crystals at high density remain a
bottleneck, due to the lack of the necessary equipments in
ordinary laboratories. We describe here
supersaturation-controlled microcrystallization and
visualization and analysis tools that can be easily used in
any laboratory. The microcrystallization conditions of the
influenza virus hemagglutinin were initially obtained with
low reproducibility, which was improved by employing a rapid
evaporation of hanging drops. Supersaturation-controlled
microcrystallization was then developed in a vapor diffusion
mode, where supersaturation was induced by evaporation in
hanging drops sequentially for durations ranging from
30 sec to 3 min, depending on the protein. It was
applied successfully to the microcrystal formation of
lysozyme, ferritin and hemagglutinin with high density.
Moreover, visualization and analysis tools were developed to
characterize the microcrystals observed by light microscopy.
The size and density distributions of microcrystals analyzed
by the tools were found to be consistent with the results of
manual analysis, further validated by high-resolution
microscopic analyses. Our supersaturation-controlled
microcrystallization and visualization and analysis tools
will provide universal access to successful XFEL studies.},
cin = {FS-CFEL-1},
ddc = {600},
cid = {I:(DE-H253)FS-CFEL-1-20120731},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621)},
pid = {G:(DE-HGF)POF3-6215},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29666410},
UT = {WOS:000430184400001},
doi = {10.1038/s41598-018-24178-5},
url = {https://bib-pubdb1.desy.de/record/417951},
}