Journal Article

PUBDB-2017-09604

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Control of type III protein secretion using a minimal genetic system

Song, M. ; Sukovich, D. J. ; Ciccarelli, L.CSSB*DESY* ; Mayr, J.CSSB*DESY* ; Fernandez-Rodriguez, J. ; Mirsky, E. A. ; Tucker, A. C. ; Gordon, D. B. ; Marlovits, T. C.CSSB*DESY* ; Voigt, C. A. (Corresponding author)

2017
Macmillan London

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Please use a persistent id in citations: doi:10.1038/ncomms14737  doi:10.3204/PUBDB-2017-09604

Abstract: Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology.

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Contributing Institute(s):

1. CSSB-UKE (CSSB-UKE)
2. CSSB-UKE-TM (CSSB-UKE-TM)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2017

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Database coverage:
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