% IMPORTANT: The following is UTF-8 encoded. This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.
@ARTICLE{Song:390512,
author = {Song, Miryoung and Sukovich, David J. and Ciccarelli,
Luciano and Mayr, Julia and Fernandez-Rodriguez, Jesus and
Mirsky, Ethan A. and Tucker, Alex C. and Gordon, D. Benjamin
and Marlovits, Thomas C. and Voigt, Christopher A.},
title = {{C}ontrol of type {III} protein secretion using a minimal
genetic system},
journal = {Nature Communications},
volume = {8},
issn = {2041-1723},
address = {London},
publisher = {Macmillan},
reportid = {PUBDB-2017-09604},
pages = {14737},
year = {2017},
abstract = {Gram-negative bacteria secrete proteins using a type III
secretion system (T3SS), which functions as a needle-like
molecular machine. The many proteins involved in T3SS
construction are tightly regulated due to its role in
pathogenesis and motility. Here, starting with the 35 kb
Salmonella pathogenicity island 1 (SPI-1), we eliminated
internal regulation and simplified the genetics by removing
or recoding genes, scrambling gene order and replacing all
non-coding DNA with synthetic genetic parts. This process
results in a 16 kb cluster that shares no sequence
identity, regulation or organizational principles with
SPI-1. Building this simplified system led to the discovery
of essential roles for an internal start site (SpaO) and
small RNA (InvR). Further, it can be controlled using
synthetic regulatory circuits, including under SPI-1
repressing conditions. This work reveals an incredible
post-transcriptional robustness in T3SS assembly and aids
its control as a tool in biotechnology.},
cin = {CSSB-UKE / CSSB-UKE-TM},
ddc = {500},
cid = {I:(DE-H253)CSSB-UKE-20141216 /
I:(DE-H253)CSSB-UKE-TM-20210520},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621)},
pid = {G:(DE-HGF)POF3-6215},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000400846400001},
pubmed = {pmid:28485369},
doi = {10.1038/ncomms14737},
url = {https://bib-pubdb1.desy.de/record/390512},
}