TY  - JOUR
AU  - Song, Miryoung
AU  - Sukovich, David J.
AU  - Ciccarelli, Luciano
AU  - Mayr, Julia
AU  - Fernandez-Rodriguez, Jesus
AU  - Mirsky, Ethan A.
AU  - Tucker, Alex C.
AU  - Gordon, D. Benjamin
AU  - Marlovits, Thomas C.
AU  - Voigt, Christopher A.
TI  - Control of type III protein secretion using a minimal genetic system
JO  - Nature Communications
VL  - 8
SN  - 2041-1723
CY  - London
PB  - Macmillan
M1  - PUBDB-2017-09604
SP  - 14737
PY  - 2017
AB  - Gram-negative bacteria secrete proteins using a type III secretion system (T3SS), which functions as a needle-like molecular machine. The many proteins involved in T3SS construction are tightly regulated due to its role in pathogenesis and motility. Here, starting with the 35 kb Salmonella pathogenicity island 1 (SPI-1), we eliminated internal regulation and simplified the genetics by removing or recoding genes, scrambling gene order and replacing all non-coding DNA with synthetic genetic parts. This process results in a 16 kb cluster that shares no sequence identity, regulation or organizational principles with SPI-1. Building this simplified system led to the discovery of essential roles for an internal start site (SpaO) and small RNA (InvR). Further, it can be controlled using synthetic regulatory circuits, including under SPI-1 repressing conditions. This work reveals an incredible post-transcriptional robustness in T3SS assembly and aids its control as a tool in biotechnology.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000400846400001
C6  - pmid:28485369
DO  - DOI:10.1038/ncomms14737
UR  - https://bib-pubdb1.desy.de/record/390512
ER  -