Journal Article

PUBDB-2015-05540

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Ceramic micro-injection molded nozzles for serial femtosecond crystallography sample delivery

Beyerlein, K.CFEL*DESY* ; Adriano, L.DESY* ; Heymann, M. ; Kirian, R.CFEL*DESY* ; Knoška, J.CFEL* ; Wilde, F.>Extern* ; Chapman, H. N.CFEL*DESY* ; Bajt, S. (Corresponding author)DESY*

2015
American Institute of Physics [S.l.]

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Please use a persistent id in citations: doi:10.1063/1.4936843

Abstract: Serial femtosecond crystallography (SFX) using X-ray Free-Electron Lasers (XFELs) allows for room temperature protein structure determination without evidence of conventional radiation damage. In this method, a liquid suspension of protein microcrystals can be delivered to the X-ray beamin vacuum as a micro-jet, which replenishes the crystals at a rate that exceeds the current XFEL pulse repetition rate. Gas dynamic virtual nozzles produce the required micrometer-sized streams by the focusing action of a coaxial sheath gas and have been shown to be effective for SFXexperiments. Here, we describe the design and characterization of such nozzles assembled from ceramic micro-injection molded outer gas-focusing capillaries. Trends of the emitted jet diameter and jet length as a function of supplied liquid and gas flow rates are measured by a fast imagingsystem. The observed trends are explained by derived relationships considering choked gas flow and liquid flow conservation. Finally, the performance of these nozzles in a SFX experiment is presented, including an analysis of the observed background.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. FS-Arbeitsgruppe (FS-ML)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. 6G3 - PETRA III (POF3-622) (POF3-622)
3. 05E13GU1 - Entwicklung eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05E13GU1) (BMBF-05E13GU1)

Experiment(s):

1. PETRA Beamline P05 (PETRA III)
2. Experiments at CFEL

Appears in the scientific report 2015

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Database coverage:
; ; ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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