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@ARTICLE{Beyerlein:291710,
author = {Beyerlein, Kenneth and Adriano, L. and Heymann, M. and
Kirian, R. and Knoška, J. and Wilde, F. and Chapman, H. N.
and Bajt, Sasa},
title = {{C}eramic micro-injection molded nozzles for serial
femtosecond crystallography sample delivery},
journal = {Review of scientific instruments},
volume = {86},
number = {12},
issn = {1089-7623},
address = {[S.l.]},
publisher = {American Institute of Physics},
reportid = {PUBDB-2015-05540},
pages = {125104 -},
year = {2015},
abstract = {Serial femtosecond crystallography (SFX) using X-ray
Free-Electron Lasers (XFELs) allows for room temperature
protein structure determination without evidence of
conventional radiation damage. In this method, a liquid
suspension of protein microcrystals can be delivered to the
X-ray beamin vacuum as a micro-jet, which replenishes the
crystals at a rate that exceeds the current XFEL pulse
repetition rate. Gas dynamic virtual nozzles produce the
required micrometer-sized streams by the focusing action of
a coaxial sheath gas and have been shown to be effective for
SFXexperiments. Here, we describe the design and
characterization of such nozzles assembled from ceramic
micro-injection molded outer gas-focusing capillaries.
Trends of the emitted jet diameter and jet length as a
function of supplied liquid and gas flow rates are measured
by a fast imagingsystem. The observed trends are explained
by derived relationships considering choked gas flow and
liquid flow conservation. Finally, the performance of these
nozzles in a SFX experiment is presented, including an
analysis of the observed background.},
cin = {FS-CFEL-1 / FS-ML},
ddc = {530},
cid = {I:(DE-H253)FS-CFEL-1-20120731 / I:(DE-H253)FS-ML-20120731},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
6G3 - PETRA III (POF3-622) / 05E13GU1 - Entwicklung eines
"Serial Femtosecond Crystallography (SFX)"-Messtandes am
Europäischen Elektronenlaser XFEL (BMBF-05E13GU1)},
pid = {G:(DE-HGF)POF3-6215 / G:(DE-HGF)POF3-6G3 /
G:(DE-H253)BMBF-05E13GU1},
experiment = {EXP:(DE-H253)P-P05-20150101 /
EXP:(DE-H253)CFEL-Exp-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000368594900072},
pubmed = {pmid:26724070},
doi = {10.1063/1.4936843},
url = {https://bib-pubdb1.desy.de/record/291710},
}