Journal Article/Journal Article

PHPPUBDB-22476

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png High-resolution protein structure determination by serial femtosecond crystallography

Boutet, S. ; Lomb, L. ; Williams, G. J. ; Barends, T. R. M. ; Aquila, A. ; Doak, R. B. ; Weierstall, U. ; DePonte, D. ; Steinbrener, J. ; Shoeman, R. L. ; Messerschmidt, M. ; Barty, A. ; White, T. A. ; Kassemeyer, S. ; Kirian, R. A. ; Seibert, M. M. ; Montanez, P. A. ; Kenney, C. ; Herbst, R. ; Hart, P. ; Pines, J. ; Haller, G. ; Gruner, S. M. ; Philipp, H. T. ; Tate, M. W. ; Hromalik, M. ; Koerner, L. J. ; van Bakel, N. ; Morse, J. ; Ghonsalves, W. ; Arnlund, D. ; Bogan, M. J. ; Caleman, C. ; Fromme, R. ; Hampton, C. Y. ; Hunter, M. S. ; Johansson, L. C. ; Katona, G. ; Kupitz, C. ; Liang, M. ; Martin, A. V. ; Nass, K. ; Redecke, L. ; Stellato, F. ; Timneanu, N. ; Wang, D. ; Zatsepin, N. A. ; Schafer, D. ; Defever, J. ; Neutze, R. ; Fromme, P. ; Spence, J. C. H. ; Chapman, H. N. ; Schlichting, I. ; DESY

2012

This record in other databases:        

Please use a persistent id in citations: doi:10.1126/science.1217737

Abstract: Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.

Keyword(s): Animals (MeSH) ; Crystallography, X-Ray: methods (MeSH) ; Lasers (MeSH) ; Muramidase: chemistry (MeSH) ; Muramidase: radiation effects (MeSH) ; Protein Conformation (MeSH) ; hen egg lysozyme ; Muramidase

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. Forschungsgruppe für strukturelle Dynamik (MPSD)

Research Program(s):

1. Experiments at CFEL (POF2-544) (POF2-544)

Experiment(s):

1. Experiments at CFEL

Appears in the scientific report 2012
Notes: First published online in Science Express on 31. May 2012. Final "paper" version published in "main" Science on 20. July 2012.

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