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000140575 1001_ $$0P:(DE-H253)PIP1009086$$aBoutet, S.$$b0
000140575 1101_ $$aDESY$$bCFEL Publications
000140575 245__ $$aHigh-resolution protein structure determination by serial femtosecond crystallography
000140575 260__ $$c2012
000140575 300__ $$a362-364
000140575 3367_ $$00$$2EndNote$$aJournal Article
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000140575 440_0 $$0PERI:(DE-600)2060783-0$$aScience$$v337$$x0036-8075$$y6092
000140575 500__ $$3Converted on 2013-05-30 09:45
000140575 500__ $$3Converted on 2013-06-21 19:21
000140575 520__ $$aStructure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.
000140575 536__ $$0G:(DE-H253)POF2-CFEL-Exp.-20130405$$aExperiments at CFEL (POF2-544)$$cPOF2-544$$fPOF II$$x0
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000140575 650_7 $$0EC 3.2.1.-$$2NLM Chemicals$$ahen egg lysozyme
000140575 650_7 $$0EC 3.2.1.17$$2NLM Chemicals$$aMuramidase
000140575 650_2 $$2MeSH$$aAnimals
000140575 650_2 $$2MeSH$$aCrystallography, X-Ray: methods
000140575 650_2 $$2MeSH$$aLasers
000140575 650_2 $$2MeSH$$aMuramidase: chemistry
000140575 650_2 $$2MeSH$$aMuramidase: radiation effects
000140575 650_2 $$2MeSH$$aProtein Conformation
000140575 693__ $$0EXP:(DE-H253)CFEL-Exp-20150101$$5EXP:(DE-H253)CFEL-Exp-20150101$$eExperiments at CFEL$$x0
000140575 7001_ $$0P:(DE-H253)PIP1008257$$aLomb, L.$$b1
000140575 7001_ $$aWilliams, G. J.$$b2
000140575 7001_ $$aBarends, T. R. M.$$b3
000140575 7001_ $$0P:(DE-H253)PIP1009066$$aAquila, A.$$b4
000140575 7001_ $$0P:(DE-H253)PIP1008250$$aDoak, R. B.$$b5
000140575 7001_ $$0P:(DE-H253)PIP1008265$$aWeierstall, U.$$b6
000140575 7001_ $$0P:(DE-H253)PIP1008249$$aDePonte, Daniel$$b7$$udesy
000140575 7001_ $$0P:(DE-H253)PIP1014108$$aSteinbrener, J.$$b8
000140575 7001_ $$aShoeman, R. L.$$b9
000140575 7001_ $$0P:(DE-H253)PIP1005807$$aMesserschmidt, M.$$b10
000140575 7001_ $$0P:(DE-H253)PIP1008245$$aBarty, A.$$b11
000140575 7001_ $$aWhite, T. A.$$b12
000140575 7001_ $$0P:(DE-H253)PIP1013003$$aKassemeyer, S.$$b13
000140575 7001_ $$aKirian, R. A.$$b14
000140575 7001_ $$aSeibert, M. M.$$b15
000140575 7001_ $$aMontanez, P. A.$$b16
000140575 7001_ $$aKenney, C.$$b17
000140575 7001_ $$aHerbst, R.$$b18
000140575 7001_ $$aHart, P.$$b19
000140575 7001_ $$aPines, J.$$b20
000140575 7001_ $$aHaller, G.$$b21
000140575 7001_ $$aGruner, S. M.$$b22
000140575 7001_ $$aPhilipp, H. T.$$b23
000140575 7001_ $$aTate, M. W.$$b24
000140575 7001_ $$aHromalik, M.$$b25
000140575 7001_ $$aKoerner, L. J.$$b26
000140575 7001_ $$0P:(DE-H253)PIP1013778$$avan Bakel, N.$$b27
000140575 7001_ $$0P:(DE-H253)PIP1010515$$aMorse, J.$$b28
000140575 7001_ $$aGhonsalves, W.$$b29
000140575 7001_ $$0P:(DE-H253)PIP1013008$$aArnlund, D.$$b30
000140575 7001_ $$aBogan, M. J.$$b31
000140575 7001_ $$0P:(DE-H253)PIP1011740$$aCaleman, C.$$b32
000140575 7001_ $$aFromme, R.$$b33
000140575 7001_ $$aHampton, C. Y.$$b34
000140575 7001_ $$aHunter, M. S.$$b35
000140575 7001_ $$aJohansson, L. C.$$b36
000140575 7001_ $$0P:(DE-H253)PIP1020897$$aKatona, G.$$b37
000140575 7001_ $$aKupitz, C.$$b38
000140575 7001_ $$0P:(DE-H253)PIP1007507$$aLiang, M.$$b39
000140575 7001_ $$aMartin, A. V.$$b40
000140575 7001_ $$0P:(DE-H253)PIP1009197$$aNass, K.$$b41
000140575 7001_ $$0P:(DE-H253)PIP1008743$$aRedecke, L.$$b42
000140575 7001_ $$0P:(DE-H253)PIP1011192$$aStellato, F.$$b43
000140575 7001_ $$0P:(DE-H253)PIP1008264$$aTimneanu, N.$$b44
000140575 7001_ $$0P:(DE-H253)PIP1011347$$aWang, D.$$b45
000140575 7001_ $$aZatsepin, N. A.$$b46
000140575 7001_ $$0P:(DE-H253)PIP1021667$$aSchafer, D.$$b47
000140575 7001_ $$aDefever, J.$$b48
000140575 7001_ $$aNeutze, R.$$b49
000140575 7001_ $$0P:(DE-H253)PIP1023170$$aFromme, P.$$b50
000140575 7001_ $$aSpence, J. C. H.$$b51
000140575 7001_ $$0P:(DE-H253)PIP1006324$$aChapman, H. N.$$b52
000140575 7001_ $$0P:(DE-H253)PIP1008260$$aSchlichting, I.$$b53
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000140575 85640 $$uhttp://www.sciencemag.org/content/337/6092/362
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000140575 9141_ $$aFirst published online in Science Express on 31. May 2012.  Final "paper" version published in "main" Science on 20. July 2012.$$y2012
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