TY  - JOUR
AU  - Boutet, S.
AU  - Lomb, L.
AU  - Williams, G. J.
AU  - Barends, T. R. M.
AU  - Aquila, A.
AU  - Doak, R. B.
AU  - Weierstall, U.
AU  - DePonte, Daniel
AU  - Steinbrener, J.
AU  - Shoeman, R. L.
AU  - Messerschmidt, M.
AU  - Barty, A.
AU  - White, T. A.
AU  - Kassemeyer, S.
AU  - Kirian, R. A.
AU  - Seibert, M. M.
AU  - Montanez, P. A.
AU  - Kenney, C.
AU  - Herbst, R.
AU  - Hart, P.
AU  - Pines, J.
AU  - Haller, G.
AU  - Gruner, S. M.
AU  - Philipp, H. T.
AU  - Tate, M. W.
AU  - Hromalik, M.
AU  - Koerner, L. J.
AU  - van Bakel, N.
AU  - Morse, J.
AU  - Ghonsalves, W.
AU  - Arnlund, D.
AU  - Bogan, M. J.
AU  - Caleman, C.
AU  - Fromme, R.
AU  - Hampton, C. Y.
AU  - Hunter, M. S.
AU  - Johansson, L. C.
AU  - Katona, G.
AU  - Kupitz, C.
AU  - Liang, M.
AU  - Martin, A. V.
AU  - Nass, K.
AU  - Redecke, L.
AU  - Stellato, F.
AU  - Timneanu, N.
AU  - Wang, D.
AU  - Zatsepin, N. A.
AU  - Schafer, D.
AU  - Defever, J.
AU  - Neutze, R.
AU  - Fromme, P.
AU  - Spence, J. C. H.
AU  - Chapman, H. N.
AU  - Schlichting, I.
AU  - DESY
TI  - High-resolution protein structure determination by serial femtosecond crystallography
JO  - Science
VL  - 337
SN  - 0036-8075
M1  - PHPPUBDB-22476
SP  - 362-364
PY  - 2012
AB  - Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.
KW  - Animals
KW  - Crystallography, X-Ray: methods
KW  - Lasers
KW  - Muramidase: chemistry
KW  - Muramidase: radiation effects
KW  - Protein Conformation
KW  - hen egg lysozyme (NLM Chemicals)
KW  - Muramidase (NLM Chemicals)
LB  - PUB:(DE-HGF)16 ; PUB:(DE-HGF)16
C6  - pmid:22653729
UR  - <Go to ISI:>//WOS:000307484300005
DO  - DOI:10.1126/science.1217737
UR  - https://bib-pubdb1.desy.de/record/140575
ER  -