Journal Article

PUBDB-2025-02190

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Model-free photon analysis of diffusion-based single-molecule FRET experiments

Terterov, I. (Corresponding author) ; Nettels, D. ; Lastiza-Male, T. ; Bartels, K.CSSB*EMBL*INFN* ; Loew, C.Extern*CSSB* ; Vancraenenbroeck, R. ; Carmel, I. ; Rosenblum, G. ; Hofmann, H. (Corresponding author)

2025
Springer Nature [London]

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Please use a persistent id in citations: doi:10.1038/s41467-025-60764-8  doi:10.3204/PUBDB-2025-02190

Abstract: Photon-by-photon analysis tools for diffusion-based single-molecule Förster resonance energy transfer (smFRET) experiments often describe protein dynamics with Markov models. However, FRET efficiencies are only projections of the conformational space such that the measured dynamics can appear non-Markovian. Model-free methods to quantify FRET efficiency fluctuations would be desirable in this case. Here, we present such an approach. We determine FRET efficiency correlation functions free of artifacts from the finite length of photon trajectories or the diffusion of molecules through the confocal volume. We show that these functions capture the dynamics of proteins from nano- to milliseconds both in simulation and experiment, which provides a rigorous validation of current model-based analysis approaches.

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Contributing Institute(s):

1. CSSB-EMBL-CL (CSSB-EMBL-CL)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2025

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