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@ARTICLE{Terterov:632562,
      author       = {Terterov, Ivan and Nettels, Daniel and Lastiza-Male, Tanya
                      and Bartels, Kim and Loew, Christian and Vancraenenbroeck,
                      Renee and Carmel, Itay and Rosenblum, Gabriel and Hofmann,
                      Hagen},
      title        = {{M}odel-free photon analysis of diffusion-based
                      single-molecule {FRET} experiments},
      journal      = {Nature Communications},
      volume       = {16},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Springer Nature},
      reportid     = {PUBDB-2025-02190},
      pages        = {5537},
      year         = {2025},
      abstract     = {Photon-by-photon analysis tools for diffusion-based
                      single-molecule Förster resonance energy transfer (smFRET)
                      experiments often describe protein dynamics with Markov
                      models. However, FRET efficiencies are only projections of
                      the conformational space such that the measured dynamics can
                      appear non-Markovian. Model-free methods to quantify FRET
                      efficiency fluctuations would be desirable in this case.
                      Here, we present such an approach. We determine FRET
                      efficiency correlation functions free of artifacts from the
                      finite length of photon trajectories or the diffusion of
                      molecules through the confocal volume. We show that these
                      functions capture the dynamics of proteins from nano- to
                      milliseconds both in simulation and experiment, which
                      provides a rigorous validation of current model-based
                      analysis approaches.},
      cin          = {CSSB-EMBL-CL},
      ddc          = {500},
      cid          = {I:(DE-H253)CSSB-EMBL-CL-20210806},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633)},
      pid          = {G:(DE-HGF)POF4-633},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40595536},
      doi          = {10.1038/s41467-025-60764-8},
      url          = {https://bib-pubdb1.desy.de/record/632562},
}