Journal Article

PUBDB-2025-01762

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png 3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomography

Schaeper, J. J.Extern* ; Kampshoff, C. A.Extern* ; Wolf, B. J. ; Roos, L. ; Michanski, S. ; Ruhwedel, T. ; Eckermann, M.Extern* ; Meyer, A. ; Jeschke, M. ; Wichmann, C. ; Moser, T.Extern* ; Salditt, T. (Corresponding author)Extern*

2025
Springer Nature [London]

This record in other databases:      

Please use a persistent id in citations: doi:10.1038/s41598-025-89431-0  doi:10.3204/PUBDB-2025-01762

Abstract: Multi-scale X-ray phase contrast tomography (XPCT) enables three-dimensional (3D), non-destructive imaging of intact small animal cochlea and apical cochlear turns. Here we report on post-mortem imaging of excised non-human primate and rodent cochleae at different $μ$-CT and nano-CT synchrotron instruments. We explore different sample embeddings, stainings and imaging regimes. Under optimized conditions of sample preparation, instrumentation, imaging protocol, and phase retrieval, high image quality and detail level can be achieved in 3D reconstructions. The showcased instrumentation and imaging protocols along with the reconstucted volumes can serve as benchmarks and reference for multi-scale microanatomy and 3D histology. The provided benchmarks and imaging protocols of this work cover a wide range of scales and are intended as augmented imaging tools for auditory research.

---

Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. FS-Proposal: I-20210562 (I-20210562) (I-20210562)
3. DFG project G:(GEPRIS)390729940 - EXC 2067: Multiscale Bioimaging: Von molekularen Maschinen zu Netzwerken erregbarer Zellen (390729940) (390729940)
4. 05K22MG1 - Methodische Entwicklung eines neuen Phasenkontrast-Tomographie Verfahrens mit holographischer Bildrekonstruktion (Holo-Tomographie). (BMBF-05K22MG1) (BMBF-05K22MG1)
5. DFG project G:(GEPRIS)317475864 - SFB 1286: Quantitative Synaptologie (317475864) (317475864)

Experiment(s):

1. PETRA Beamline P10 (PETRA III)

Appears in the scientific report 2025

---

Database coverage:
; ; ; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---