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@ARTICLE{Schaeper:628959,
author = {Schaeper, Jannis J. and Kampshoff, Christoph A. and Wolf,
Bettina J. and Roos, Lennart and Michanski, Susann and
Ruhwedel, Torben and Eckermann, Marina and Meyer, Alexander
and Jeschke, Marcus and Wichmann, Carolin and Moser, Tobias
and Salditt, Tim},
title = {3{D} virtual histology of rodent and primate cochleae with
multi-scale phase-contrast {X}-ray tomography},
journal = {Scientific reports},
volume = {15},
number = {1},
issn = {2045-2322},
address = {[London]},
publisher = {Springer Nature},
reportid = {PUBDB-2025-01762},
pages = {7933},
year = {2025},
abstract = {Multi-scale X-ray phase contrast tomography (XPCT) enables
three-dimensional (3D), non-destructive imaging of intact
small animal cochlea and apical cochlear turns. Here we
report on post-mortem imaging of excised non-human primate
and rodent cochleae at different $μ$-CT and nano-CT
synchrotron instruments. We explore different sample
embeddings, stainings and imaging regimes. Under optimized
conditions of sample preparation, instrumentation, imaging
protocol, and phase retrieval, high image quality and detail
level can be achieved in 3D reconstructions. The showcased
instrumentation and imaging protocols along with the
reconstucted volumes can serve as benchmarks and reference
for multi-scale microanatomy and 3D histology. The provided
benchmarks and imaging protocols of this work cover a wide
range of scales and are intended as augmented imaging tools
for auditory research.},
cin = {DOOR ; HAS-User},
ddc = {600},
cid = {I:(DE-H253)HAS-User-20120731},
pnm = {6G3 - PETRA III (DESY) (POF4-6G3) / FS-Proposal: I-20210562
(I-20210562) / DFG project G:(GEPRIS)390729940 - EXC 2067:
Multiscale Bioimaging: Von molekularen Maschinen zu
Netzwerken erregbarer Zellen (390729940) / 05K22MG1 -
Methodische Entwicklung eines neuen
Phasenkontrast-Tomographie Verfahrens mit holographischer
Bildrekonstruktion (Holo-Tomographie). (BMBF-05K22MG1) / DFG
project G:(GEPRIS)317475864 - SFB 1286: Quantitative
Synaptologie (317475864)},
pid = {G:(DE-HGF)POF4-6G3 / G:(DE-H253)I-20210562 /
G:(GEPRIS)390729940 / G:(DE-Ds200)BMBF-05K22MG1 /
G:(GEPRIS)317475864},
experiment = {EXP:(DE-H253)P-P10-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {40050327},
UT = {WOS:001439325200009},
doi = {10.1038/s41598-025-89431-0},
url = {https://bib-pubdb1.desy.de/record/628959},
}