Journal Article

PUBDB-2025-01457

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Proteorhodopsin insights into the molecular mechanism of vectorial proton transport

Bukhdruker, S. ; Gushchin, I. ; Shevchenko, V. ; Kovalev, K.EMBL* ; Polovinkin, V. ; Tsybrov, F. ; Astashkin, R. ; Alekseev, A. ; Mikhaylov, A. ; Bukhalovich, S. ; Bratanov, D. ; Ryzhykau, Y. ; Kuklina, D. ; Caramello, N. ; Rokitskaya, T. ; Antonenko, Y. ; Rulev, M. ; Stoev, C. ; Zabelskii, D. ; Round, E. ; Rogachev, A. ; Borshchevskiy, V. ; Ghai, R. ; Bourenkov, G.XFEL.EU*EMBL* ; Zeghouf, M. ; Cherfils, J. ; Engelhard, M. ; Chizhov, I. ; Rodriguez-Valera, F. ; Bamberg, E. ; Gordeliy, V. (Corresponding author)Extern*EMBL*

2025
Assoc. Washington, DC [u.a.]

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Please use a persistent id in citations: doi:10.1126/sciadv.adu5303  doi:10.3204/PUBDB-2025-01457

Abstract: Bacterial proton pumps, proteorhodopsins (PRs), are a major group of light-driven membrane proteins found in marine bacteria. They are functionally and structurally distinct from archaeal and eukaryotic proton pumps. To elucidate the proton transfer mechanism by PRs and understand the differences to nonbacterial pumps on a molecular level, high-resolution structures of PRs’ functional states are needed. In this work, we have determined atomic-resolution structures of MAR, a PR from marine actinobacteria, in various functional states, notably the challenging late O intermediate state. These data and information from recent atomic-resolution structures on an archaeal outward proton pump bacteriorhodopsin and bacterial inward proton pump xenorhodopsin allow for deducing key universal elements for light-driven proton pumping. First, long hydrogen-bonded chains characterize proton pathways. Second, short hydrogen bonds allow proton storage and inhibit their backflow. Last, the retinal Schiff base is the active proton donor and acceptor to and from hydrogen-bonded chains.

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Contributing Institute(s):

1. EMBL-User (EMBL-User)
2. EMBL (EMBL)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)

Experiment(s):

1. PETRA Beamline P14 (PETRA III)

Appears in the scientific report 2025

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