Journal Article

PUBDB-2025-00570

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Resilience and charge-dependent fibrillation of functional amyloid: Interactions of Pseudomonas biofilm-associated FapB and FapC amyloids

Golan, N. ; Parizat, A. ; Tabachnikov, O. ; Barnea, E. ; Olsen, W. P. ; Otzen, D. E. ; Landau, M. (Corresponding author)CSSB*EMBL*DESY*

2025
American Soc. for Biochemistry and Molecular Biology Bethesda, MD

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Please use a persistent id in citations: doi:10.1016/j.jbc.2024.108096  doi:10.3204/PUBDB-2025-00570

Abstract: FapC and FapB are biofilm-associated amyloids involved in the virulence of Pseudomonas and other bacteria. We herein demonstrate their exceptional thermal and chemical resilience, suggesting that their biofilm structures might withstand standard sterilization, thereby contributing to the persistence of Pseudomonas aeruginosa infections. Our findings also underscore the impact of environmental factors on functional amyloid in Pseudomonas (Fap) proteins, suggesting that orthologs in different Pseudomonas strains adapt to specific environments and roles. Challenging previous assumptions about a simple nucleation role for FapB in promoting FapC aggregation, the study shows a significant influence of FapC on FapB aggregation. The interaction between these FapB and FapC is intricate: FapB stabilizes FapC fibrils, while FapC slows down FapB fibrillation but can still serve as a cross-seeding template. This complex interplay is the key to understanding their roles in bacterial biofilms. Furthermore, the study highlights distinct differences between Fap and Escherichia coli's CsgA (curli) amyloid, where CsgB assumes a simple unidirectional role in nucleating CsgA fibrillation, emphasizing the importance of a comprehensive understanding of various amyloid systems. This knowledge is vital for developing effective intervention strategies against bacterial infections and leveraging the unique properties of these amyloids in technological applications such as novel bionanomaterials or protective coatings.

Note: M. L. acknowledges research support from the Israel Science Foundation, Grant No. 2111/20, Israel, the Cure Alzheimer’s Fund, USA, the Forschungskooperation Niedersachsen–Israel, Volkswagenstiftung, No: 76251-4659/2022 (ZN 4042), Germany, and the Russell Berrie Nanotechnology Institue (RBNI), Israel.

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Contributing Institute(s):

1. FPF Functional Protein Fibrils (CSSB-F)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2025

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Preprint Golan, N. ; Parizat, A. ; Tabachnikov, O. ; Barnea, E. ; Olsen, W. P. ; Otzen, D. E. ; Landau, M. (Corresponding author)CSSB*EMBL*DESY*
Resilience and Charge-Dependent Fibrillation of Functional Amyloids: Interactions of Pseudomonas Biofilm-Associated FapB and FapC
[10.1101/2024.02.14.580233]     Files BibTeX | EndNote: XML, Text | RIS

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