Journal Article

PUBDB-2024-07871

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structure of Protein Cage Supercrystals revealed by Angular X-ray Cross-Correlation Analysis

Ngoi, K. H.DESY* ; Lang, L.Extern* ; Kim, Y. Y.Extern* ; Mucke, N.Extern* ; Hinsley, G.Extern*DESY* ; Dongwon, K. ; Ruetten, M.Extern*EMBL* ; Ruffer, M.Extern* ; Yadav, V.Extern* ; Wagler, H.Extern* ; Katenkamp, T.Extern* ; Perbandt, M.Extern*XFEL.EU* ; Khadiev, A.Extern*DESY* ; Beck, T. (Corresponding author)Extern* ; Vartaniants, I. (Corresponding author)DESY*

2025
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/sstr.202400684  doi:10.3204/PUBDB-2024-07871

Abstract: Biohybrid supercrystals are highly ordered three-dimensional (3D) assemblies of protein nanocages, offering versatile structural designs through their ability to encapsulate various inorganic nanoparticles. By loading nanoparticles into these nanocages, diverse and complex superstructures can be engineered. In this study, we investigate individual biohybrid supercrystals using small angle X-ray diffraction (SAXD). We employ Angular X-ray Cross-Correlation Analysis (AXCCA) to the intensity distribution in 3D reciprocal space, enabling the determination of the unit cell parameters of the superlattice. Encapsulated nanoparticles serve as effective X-ray scattering markers, enabling precise localization of protein nanocages within the superlattice. The arrangement of nanoparticles in the unit cell is validated by comparing the experimental and calculated radial intensity profile. Our findings confirm the superlattice structures of unitary protein-nanoparticle composites, binary composites (including homobinary and heterobinary designs), and supercrystals with core-shell morphologies. Furthermore, single-grain and twin-domain structures are identified, demonstrating the potential of this technique for defect characterization and crystal engineering.

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Contributing Institute(s):

1. DOOR-User (DOOR ; HAS-User)
2. Experimentebetreuung PETRA III (FS-PET-D)
3. FS-Photon Science (FS-PS)

Research Program(s):

1. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. AIM, DFG project G:(GEPRIS)390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
4. FS-Proposal: I-20230919 (I-20230919) (I-20230919)
5. GRK 2536 - GRK 2536: Hybridstrukturen auf der Nanometerskala: Chemische Konzepte zur Herstellung heterogener Nanostrukturen mit anisotropen Materialeigenschaften (NANOHYBRID) (408076438) (408076438)

Experiment(s):

1. PETRA Beamline P23 (PETRA III)

Appears in the scientific report 2025

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