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@ARTICLE{Ngoi:619736,
author = {Ngoi, Kuan Hoon and Lang, Laurin and Kim, Young Yong and
Mucke, Niklas and Hinsley, Gerard and Dongwon, Kim and
Ruetten, Michael and Ruffer, Maximilian and Yadav, Varnika
and Wagler, Henrike and Katenkamp, Tobias and Perbandt,
Markus and Khadiev, Azat and Beck, Tobias and Vartaniants,
Ivan},
title = {{S}tructure of {P}rotein {C}age {S}upercrystals revealed by
{A}ngular {X}-ray {C}ross-{C}orrelation {A}nalysis},
journal = {Small structures},
volume = {6},
number = {8},
issn = {2688-4062},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {PUBDB-2024-07871},
pages = {2400684},
year = {2025},
abstract = {Biohybrid supercrystals are highly ordered
three-dimensional (3D) assemblies of protein nanocages,
offering versatile structural designs through their ability
to encapsulate various inorganic nanoparticles. By loading
nanoparticles into these nanocages, diverse and complex
superstructures can be engineered. In this study, we
investigate individual biohybrid supercrystals using small
angle X-ray diffraction (SAXD). We employ Angular X-ray
Cross-Correlation Analysis (AXCCA) to the intensity
distribution in 3D reciprocal space, enabling the
determination of the unit cell parameters of the
superlattice. Encapsulated nanoparticles serve as effective
X-ray scattering markers, enabling precise localization of
protein nanocages within the superlattice. The arrangement
of nanoparticles in the unit cell is validated by comparing
the experimental and calculated radial intensity profile.
Our findings confirm the superlattice structures of unitary
protein-nanoparticle composites, binary composites
(including homobinary and heterobinary designs), and
supercrystals with core-shell morphologies. Furthermore,
single-grain and twin-domain structures are identified,
demonstrating the potential of this technique for defect
characterization and crystal engineering.},
cin = {DOOR ; HAS-User / FS-PET-D / FS-PS},
ddc = {540},
cid = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)FS-PET-D-20190712
/ I:(DE-H253)FS-PS-20131107},
pnm = {632 - Materials – Quantum, Complex and Functional
Materials (POF4-632) / 6G3 - PETRA III (DESY) (POF4-6G3) /
AIM, DFG project G:(GEPRIS)390715994 - EXC 2056: CUI:
Advanced Imaging of Matter (390715994) / FS-Proposal:
I-20230919 (I-20230919) / GRK 2536 - GRK 2536:
Hybridstrukturen auf der Nanometerskala: Chemische Konzepte
zur Herstellung heterogener Nanostrukturen mit anisotropen
Materialeigenschaften (NANOHYBRID) (408076438)},
pid = {G:(DE-HGF)POF4-632 / G:(DE-HGF)POF4-6G3 /
G:(GEPRIS)390715994 / G:(DE-H253)I-20230919 /
G:(GEPRIS)408076438},
experiment = {EXP:(DE-H253)P-P23-20150101},
typ = {PUB:(DE-HGF)16},
doi = {10.1002/sstr.202400684},
url = {https://bib-pubdb1.desy.de/record/619736},
}