Journal Article

PUBDB-2024-07018

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Salt induced slowdown of kinetics and dynamics during thermal gelation of egg-yolk

Anthuparambil, N. D. (Corresponding author)Extern* ; Timmermann, S.Extern*XFEL.EU* ; Dargasz, M.Extern*XFEL.EU* ; Retzbach, S.Extern* ; Senft, M. D.Extern*EMBL* ; Begam, N.Extern* ; Ragulskaya, A.Extern*XFEL.EU* ; Paulus, M.Extern* ; Zhang, F.Extern* ; Westermeier, F.XFEL.EU*DESY* ; Sprung, M.XFEL.EU*DESY* ; Schreiber, F.Extern* ; Gutt, C. (Corresponding author)Extern*XFEL.EU*

2024
American Institute of Physics Melville, NY

This record in other databases:      

Please use a persistent id in citations: doi:10.1063/5.0219004  doi:10.3204/PUBDB-2024-07018

Abstract: We investigated the effect of the NaCl concentration (0.3–2M) on the structure and dynamics of hen egg yolk at room temperature andduring thermal gelation at temperatures in the range of 66–90 ○C utilizing low-dose x-ray photon correlation spectroscopy in ultra-smallangle x-ray scattering geometry. With an increase in the salt concentration, we observe progressive structural and dynamic changes at roomtemperature, indicating the disruption of yolk components such as yolk-granules and yolk-plasma proteins. Temperature- and salt-dependentstructural and dynamic investigations suggest a delay in the gel formation and aggregation of yolk low-density lipoproteins with increasingionic strength. However, the time–temperature superposition relationship observed in all samples suggests an identical mechanism underlyingprotein aggregation–gelation with a temperature-dependent reaction rate. The sol–gel transition time extracted from kinetic and dynamicinformation follows Arrhenius’s behavior, and the activation energy (460 kJ/mol) is found to be independent of the salt concentration.

Note: DFG (Grant No. NFDI 40/1)

---

Contributing Institute(s):

1. PETRA-S (FS-PETRA-S)
2. DOOR-User (DOOR ; HAS-User)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. 05K19PS1 - Instrumentierung, um die Dynamik von biologischen Proben mit Korrelationsspektroskopie zu messen. (BMBF-05K19PS1) (BMBF-05K19PS1)
4. 05K20PSA - Verbundprojekt 05K2020 - 2019-06075 Protein-Dyn: Dynamik von Proteinen in Lösungen auf multiplen Längen und Zeitskalen (Teilprojekt 1) (BMBF-05K20PSA) (BMBF-05K20PSA)
5. 05K22PS1 - Schnelle Korrelationsspektroskopie an der ESRF-EBS (BMBF-05K22PS1) (BMBF-05K22PS1)
6. 05K20VTA - Verbundprojekt 05K2020 - 2019-06075 Protein-Dyn: Dynamik von Proteinen in Lösungen auf multiplen Längen und Zeitskalen (Teilprojekt 2) (BMBF-05K20VTA) (BMBF-05K20VTA)
7. FS-Proposal: II-20210008 (II-20210008) (II-20210008)

Experiment(s):

1. PETRA Beamline P10 (PETRA III)

Appears in the scientific report 2024

---

Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; National-Konsortium ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---