Journal Article

PUBDB-2024-05938

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Raynals , an online tool for the analysis of dynamic light scattering

Burastero, O.Extern*CSSB*EMBL* ; Draper-Barr, G.Extern*CSSB*XFEL.EU* ; Raynal, B. ; Chevreuil, M. ; England, P. ; Garcia Alai, M. (Corresponding author)Extern*CSSB*EMBL*

2023
Wiley Bognor Regis

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Please use a persistent id in citations: doi:10.1107/S2059798323004862  doi:10.3204/PUBDB-2024-05938

Abstract: Dynamic light scattering (DLS) is routinely employed to assess the homogeneity and size-distribution profile of samples containing microscopic particles in suspension or solubilized polymers. In this work, Raynals, user-friendly software for the analysis of single-angle DLS data that uses the Tikhonov–Phillips regularization, is introduced. Its performance is evaluated on simulated and experimental data generated by different DLS instruments for several proteins and gold nanoparticles. DLS data can easily be misinterpreted and the simulation tools available in Raynals allow the limitations of the measurement and its resolution to be understood. It was designed as a tool to address the quality control of biological samples during sample preparation and optimization and it helps in the detection of aggregates, showing the influence of large particles. Lastly, Raynals provides flexibility in the way that the data are presented, allows the export of publication-quality figures, is free for academic use and can be accessed online on the eSPC data-analysis platform at https://spc.embl-hamburg.de/.Keywords: Raynals; dynamic light scattering; molecular biophysics; online data analysis; protein quality control; hydrodynamic radius; size distribution; software.

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Contributing Institute(s):

1. CSSB-CF-SPC (CSSB-CF-SPC)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)
2. MOSBRI - MOlecular-Scale Biophysics Research Infrastructure (101004806) (101004806)

Experiment(s):

1. No specific instrument

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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