Journal Article

PUBDB-2024-00585

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Flagellin lysine methyltransferase FliB catalyzes a [4Fe-4S] mediated methyl transfer reaction

Wang, C.CSSB*HZI* ; Nehls, C.CSSB*EMBL* ; Baabe, D. ; Burghaus, O. ; Hurwitz, R. ; Gutsmann, T.CSSB*EMBL* ; Bröring, M. ; Kolbe, M. (Corresponding author)CSSB*EMBL*

2021
PLoS Lawrence, Kan.

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Please use a persistent id in citations: doi:10.1371/journal.ppat.1010052  doi:10.3204/PUBDB-2024-00585

Abstract: The methyltransferase FliB posttranslationally modifies surface-exposed ɛ-N-lysine residues of flagellin, the protomer of the flagellar filament in Salmonella enterica (S. enterica). Flagellin methylation, reported originally in 1959, was recently shown to enhance host cell adhesion and invasion by increasing the flagellar hydrophobicity. The role of FliB in this process, however, remained enigmatic. In this study, we investigated the properties and mechanisms of FliB from S. enterica in vivo and in vitro. We show that FliB is an S-adenosylmethionine (SAM) dependent methyltransferase, forming a membrane associated oligomer that modifies flagellin in the bacterial cytosol. Using X-band electron paramagnetic resonance (EPR) spectroscopy, zero-field 57Fe Mössbauer spectroscopy, methylation assays and chromatography coupled mass spectrometry (MS) analysis, we further found that FliB contains an oxygen sensitive [4Fe-4S] cluster that is essential for the methyl transfer reaction and might mediate a radical mechanism. Our data indicate that the [4Fe-4S] cluster is coordinated by a cysteine rich motif in FliB that is highly conserved among multiple genera of the Enterobacteriaceae family.

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Contributing Institute(s):

1. CSSB-HZI-MK (CSSB-HZI-MK)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. FUTURE T3SS - Bacterial effector secretion: Function and Architecture of the Type 3 Secretion System (311374) (311374)

Experiment(s):

1. PETRA Beamline P11 (PETRA III)
2. PETRA Beamline P12 (PETRA III)

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