Journal Article

PUBDB-2024-00104

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons

del Toro, D. ; Carrasquero-Ordaz, M. A. ; Chu, A. ; Ruff, T. ; Shahin, M. ; Jackson, V. A. ; Chavent, M. ; Berbeira-Santana, M. ; Seyit-Bremer, G. ; Brignani, S. ; Kaufmann, R.CSSB* ; Lowe, E.Extern* ; Klein, R. (Corresponding author) ; Seiradake, E. (Corresponding author)

2020
Elsevier New York, NY

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Please use a persistent id in citations: doi:10.1016/j.cell.2019.12.014  doi:10.3204/PUBDB-2024-00104

Abstract: Teneurins are ancient metazoan cell adhesion receptors that control brain development and neuronal wiring in higher animals. The extracellular C terminus binds the adhesion GPCR Latrophilin, forming a trans-cellular complex with synaptogenic functions. However, Teneurins, Latrophilins, and FLRT proteins are also expressed during murine cortical cell migration at earlier developmental stages. Here, we present crystal structures of Teneurin-Latrophilin complexes that reveal how the lectin and olfactomedin domains of Latrophilin bind across a spiraling beta-barrel domain of Teneurin, the YD shell. We couple structure-based protein engineering to biophysical analysis, cell migration assays, and in utero electroporation experiments to probe the importance of the interaction in cortical neuron migration. We show that binding of Latrophilins to Teneurins and FLRTs directs the migration of neurons using a contact repulsion-dependent mechanism. The effect is observed with cell bodies and small neurites rather than their processes. The results exemplify how a structure-encoded synaptogenic protein complex is also used for repulsive cell guidance.

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Contributing Institute(s):

1. CSSB-UHH-RK (CSSB-UHH-RK)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Experiment(s):

1. No specific instrument

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 60 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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