Journal Article

PUBDB-2022-00379

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Single-Molecule FRET of Membrane Transport Proteins

Bartels, K.CSSB*EMBL*DESY* ; Lasitza-Male, T. ; Hofmann, H. (Corresponding author) ; Loew, C. (Corresponding author)CSSB*DESY*

2021
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/cbic.202100106  doi:10.3204/PUBDB-2022-00379

Abstract: Uncovering the structure and function of biomolecules is a fundamental goal in structural biology. Membrane-embedded transport proteins are ubiquitous in all kingdoms of life. Despite structural flexibility, their mechanisms are typically studied by ensemble biochemical methods or by static high-resolution structures, which complicate a detailed understanding of their dynamics. Here, we review the recent progress of single molecule Förster Resonance Energy Transfer (smFRET) in determining mechanisms and timescales of substrate transport across membranes. These studies do not only demonstrate the versatility and suitability of state-of-the-art smFRET tools for studying membrane transport proteins but they also highlight the importance of membrane mimicking environments in preserving the function of these proteins. The current achievements advance our understanding of transport mechanisms and have the potential to facilitate future progress in drug design.

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Contributing Institute(s):

1. CSSB-EMBL (CSSB-EMBL)
2. FS-Photon Science (FS-PS)
3. CSSB-EMBL-CL (CSSB-EMBL-CL)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2021

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Chemical Reactions ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Essential Science Indicators ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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