Journal Article

PUBDB-2021-01823

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Synchronous RNA conformational changes trigger ordered phase transitions in crystals

Ramakrishnan, S. ; Stagno, J.Extern*XFEL.EU* ; Conrad, C. E.Extern*XFEL.EU* ; Ding, J.Extern*XFEL.EU* ; Yu, P.Extern*XFEL.EU* ; Bhandari, Y. R. ; Lee, Y.-T. ; Pauly, G. ; Yefanov, O.CFEL*EMBL*DESY* ; Wiedorn, M. O.CFEL*XFEL.EU* ; Knoska, J.CFEL*XFEL.EU*DESY* ; Oberthür, D.CFEL*DESY* ; White, T.CFEL*DESY* ; Barty, A.CFEL*DESY* ; Mariani, V.CFEL*XFEL.EU*DESY* ; Li, C.CFEL*XFEL.EU*DESY* ; Brehm, W.CFEL*DESY* ; Heinz, W. F. ; Magidson, V. ; Lockett, S. ; Hunter, M.Extern*XFEL.EU* ; Boutet, S.Extern* ; Zatsepin, N.Extern*XFEL.EU* ; Zuo, X. ; Grant, T.Extern* ; Pandey, S.Extern*XFEL.EU* ; Schmidt, M.Extern*XFEL.EU* ; Spence, J. C. H.CFEL*Extern* ; Chapman, H. N.CFEL*DESY* ; Wang, Y.-X. (Corresponding author)Extern*

2021
Nature Publishing Group UK [London]

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Please use a persistent id in citations: doi:10.1038/s41467-021-21838-5  doi:10.3204/PUBDB-2021-01823

Abstract: Time-resolved studies of biomacromolecular crystals have been limited to systems involving only minute conformational changes within the same lattice. Ligand-induced changes greater than several angstroms, however, are likely to result in solid-solid phase transitions, which require a detailed understanding of the mechanistic interplay between conformational and lattice transitions. Here we report the synchronous behavior of the adenine riboswitch aptamer RNA in crystal during ligand-triggered isothermal phase transitions. Direct visualization using polarized video microscopy and atomic force microscopy shows that the RNA molecules undergo cooperative rearrangements that maintain lattice order, whose cell parameters change distinctly as a function of time. The bulk lattice order throughout the transition is further supported by time-resolved diffraction data from crystals using an X-ray free electron laser. The synchronous molecular rearrangements in crystal provide the physical basis for studying large conformational changes using time-resolved crystallography and micro/nanocrystals.

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Contributing Institute(s):

1. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
2. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. AIM - CUI: Advanced Imaging of Matter (390715994) (390715994)
3. Leibniz Preis - Leibiz Programm 2015: Prof. Dr. Henry N. Chapman (DFG-Leibniz-2015-Chapman) (DFG-Leibniz-2015-Chapman)

Experiment(s):

1. Experiments at CFEL
2. Measurement at external facility

Appears in the scientific report 2021

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