Journal Article

PUBDB-2021-00539

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol

Chabloz, A.DESY* ; Schaefer, J. V. ; Kozieradzki, I. ; Cronin, S. J. F. ; Strebinger, D. ; Macaluso, F. ; Wald, J. ; Rabbitts, T. H. ; Plückthun, A. ; Marlovits, T. (Corresponding author)CSSB*DESY* ; Penninger, J. M. (Corresponding author)

2020
Springer Nature London

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Please use a persistent id in citations: doi:10.1038/s42003-020-1072-4  doi:10.3204/PUBDB-2021-00539

Abstract: Protein-based affinity reagents (like antibodies or alternative binding scaffolds) offer wide-ranging applications for basic research and therapeutic approaches. However, whereas small chemical molecules efficiently reach intracellular targets, the delivery of macromolecules into the cytosol of cells remains a major challenge; thus cytosolic applications of protein-based reagents are rather limited. Some pathogenic bacteria have evolved a conserved type III secretion system (T3SS) which allows the delivery of effector proteins into eukaryotic cells. Here, we enhance the T3SS of an avirulent strain of Salmonella typhimurium to reproducibly deliver multiple classes of recombinant proteins into eukaryotic cells. The efficacy of the system is probed with both DARPins and monobodies to functionally inhibit the paradigmatic and largely undruggable RAS signaling pathway. Thus, we develop a bacterial secretion system for potent cytosolic delivery of therapeutic macromolecules.

Keyword(s): Bacterial Proteins: genetics (MeSH) ; Bacterial Proteins: metabolism (MeSH) ; Carrier Proteins: genetics (MeSH) ; Carrier Proteins: metabolism (MeSH) ; Cytosol: metabolism (MeSH) ; HCT116 Cells (MeSH) ; HeLa Cells (MeSH) ; Humans (MeSH) ; Neoplasm Proteins: genetics (MeSH) ; Neoplasm Proteins: metabolism (MeSH) ; Recombinant Proteins: genetics (MeSH) ; Recombinant Proteins: metabolism (MeSH) ; Salmonella typhimurium: genetics (MeSH) ; Salmonella typhimurium: growth & development (MeSH) ; Salmonella typhimurium: metabolism (MeSH) ; Type III Secretion Systems: genetics (MeSH) ; Type III Secretion Systems: metabolism (MeSH) ; Bacterial Proteins ; Carrier Proteins ; Neoplasm Proteins ; Recombinant Proteins ; Type III Secretion Systems

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Contributing Institute(s):

1. CSSB-UKE (CSSB-UKE)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2020

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