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@ARTICLE{Chabloz:454465,
      author       = {Chabloz, Antoine and Schaefer, Jonas V. and Kozieradzki,
                      Ivona and Cronin, Shane J. F. and Strebinger, Daniel and
                      Macaluso, Francesca and Wald, Jiri and Rabbitts, Terence H.
                      and Plückthun, Andreas and Marlovits, Thomas and Penninger,
                      Josef M.},
      title        = {{S}almonella-based platform for efficient delivery of
                      functional binding proteins to the cytosol},
      journal      = {Communications biology},
      volume       = {3},
      number       = {1},
      issn         = {2399-3642},
      address      = {London},
      publisher    = {Springer Nature},
      reportid     = {PUBDB-2021-00539},
      pages        = {342},
      year         = {2020},
      abstract     = {Protein-based affinity reagents (like antibodies or
                      alternative binding scaffolds) offer wide-ranging
                      applications for basic research and therapeutic approaches.
                      However, whereas small chemical molecules efficiently reach
                      intracellular targets, the delivery of macromolecules into
                      the cytosol of cells remains a major challenge; thus
                      cytosolic applications of protein-based reagents are rather
                      limited. Some pathogenic bacteria have evolved a conserved
                      type III secretion system (T3SS) which allows the delivery
                      of effector proteins into eukaryotic cells. Here, we enhance
                      the T3SS of an avirulent strain of Salmonella typhimurium to
                      reproducibly deliver multiple classes of recombinant
                      proteins into eukaryotic cells. The efficacy of the system
                      is probed with both DARPins and monobodies to functionally
                      inhibit the paradigmatic and largely undruggable RAS
                      signaling pathway. Thus, we develop a bacterial secretion
                      system for potent cytosolic delivery of therapeutic
                      macromolecules.},
      keywords     = {Bacterial Proteins: genetics / Bacterial Proteins:
                      metabolism / Carrier Proteins: genetics / Carrier Proteins:
                      metabolism / Cytosol: metabolism / HCT116 Cells / HeLa Cells
                      / Humans / Neoplasm Proteins: genetics / Neoplasm Proteins:
                      metabolism / Recombinant Proteins: genetics / Recombinant
                      Proteins: metabolism / Salmonella typhimurium: genetics /
                      Salmonella typhimurium: growth $\&$ development / Salmonella
                      typhimurium: metabolism / Type III Secretion Systems:
                      genetics / Type III Secretion Systems: metabolism /
                      Bacterial Proteins (NLM Chemicals) / Carrier Proteins (NLM
                      Chemicals) / Neoplasm Proteins (NLM Chemicals) / Recombinant
                      Proteins (NLM Chemicals) / Type III Secretion Systems (NLM
                      Chemicals)},
      cin          = {CSSB-UKE},
      ddc          = {570},
      cid          = {I:(DE-H253)CSSB-UKE-20141216},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621)},
      pid          = {G:(DE-HGF)POF3-6215},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32620833},
      UT           = {WOS:000549964800005},
      doi          = {10.1038/s42003-020-1072-4},
      url          = {https://bib-pubdb1.desy.de/record/454465},
}