Journal Article

PUBDB-2021-00463

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protein-crystal detection with a compact multimodal multiphoton microscope

Cheng, Q.-d. ; Chung, H.-Y. ; Schubert, R. ; Chia, S.-H. ; Falke, S. ; Mudogo, C. N. ; Kärtner, F. (Corresponding author)CFEL*DESY* ; Chang, G. (Corresponding author)CFEL*Extern* ; Betzel, C. (Corresponding author)Extern*

2020
Springer Nature London

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Please use a persistent id in citations: doi:10.1038/s42003-020-01275-8  doi:10.3204/PUBDB-2021-00463

Abstract: There is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, multiphoton microscope, driven by a fiber-based ultrafast laser, enabling excitation wavelengths at 775 nm and 1300 nm for nonlinear optical imaging, which simultaneously records second-harmonic generation, third-harmonic generation and three-photon excited ultraviolet fluorescence to identify and detect protein crystals with high sensitivity. The instrument serves as a valuable and important tool supporting sample scoring and sample optimization in biomolecular crystallography, which we hope will increase the capabilities and productivity of serial diffraction data collection in the future.

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Contributing Institute(s):

1. FS-CFEL-1 Fachgruppe BMX (FS-CFEL-1-BMX)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
3. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
4. VH-VI-419 - Dynamic Pathways in Multidimensional Landscapes (VH-VI-419) (VH-VI-419)
5. VH-NG-804 - Towards Laboratory-Based Ultrafast Bright EUV and X-ray Sources (2015_IVF-VH-NG-804) (2015_IVF-VH-NG-804)
6. HCJRG-201 - Advanced Laser Technologies for Ultrafast Spectroscopy of Quantum Materials (2015_IFV-HCJRG-201) (2015_IFV-HCJRG-201)

Experiment(s):

1. No specific instrument

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