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000454378 1001_ $$aCheng, Qing-di$$b0
000454378 245__ $$aProtein-crystal detection with a compact multimodal multiphoton microscope
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000454378 520__ $$aThere is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, multiphoton microscope, driven by a fiber-based ultrafast laser, enabling excitation wavelengths at 775 nm and 1300 nm for nonlinear optical imaging, which simultaneously records second-harmonic generation, third-harmonic generation and three-photon excited ultraviolet fluorescence to identify and detect protein crystals with high sensitivity. The instrument serves as a valuable and important tool supporting sample scoring and sample optimization in biomolecular crystallography, which we hope will increase the capabilities and productivity of serial diffraction data collection in the future.
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000454378 7001_ $$aChung, Hsiang-Yu$$b1
000454378 7001_ $$aSchubert, Robin$$b2
000454378 7001_ $$aChia, Shih-Hsuan$$b3
000454378 7001_ $$aFalke, Sven$$b4
000454378 7001_ $$aMudogo, Celestin Nzanzu$$b5
000454378 7001_ $$0P:(DE-H253)PIP1013198$$aKärtner, Franz$$b6$$eCorresponding author
000454378 7001_ $$0P:(DE-H253)PIP1017633$$aChang, Guoqing$$b7$$eCorresponding author
000454378 7001_ $$0P:(DE-H253)PIP1002913$$aBetzel, Christian$$b8$$eCorresponding author
000454378 773__ $$0PERI:(DE-600)2919698-X$$a10.1038/s42003-020-01275-8$$gVol. 3, no. 1, p. 569$$n1$$p569$$tCommunications biology$$v3$$x2399-3642$$y2020
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