Journal Article

PUBDB-2020-03502

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png An advanced workflow for single particle imaging with the limited data at an X-ray free-electron laser

Assalauova, D.XFEL.EU*DESY* ; Kim, Y. Y.XFEL.EU*DESY* ; Bobkov, S. ; Khubbutdinov, R.XFEL.EU*DESY* ; Rose, M.Extern*XFEL.EU* ; Alvarez, R.Extern*XFEL.EU* ; Andreasson, J.Extern* ; Balaur, E.Extern*XFEL.EU* ; Contreras, A. ; DeMirci, H.Extern*XFEL.EU* ; Gelisio, L. ; Hajdu, J.Extern* ; Hunter, M. S.XFEL.EU* ; Kurta, R. P.XFEL.EU* ; Li, H. ; McFadden, M. ; Nazari, R. ; Schwander, P.Extern*XFEL.EU* ; Teslyuk, A. ; Walter, P. ; Xavier, P. L. ; Yoon, C. H.Extern* ; Zaare, S. ; Ilyin, V. A. ; Kirian, R.Extern*XFEL.EU* ; Hogue, B. G. ; Aquila, A.Extern* ; Vartanyants, I. A. (Corresponding author)XFEL.EU*DESY*

2020
Chester

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Please use a persistent id in citations: doi:10.1107/S2052252520012798  doi:10.3204/PUBDB-2020-03502

Abstract: An improved analysis for single-particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the Atomic, Molecular and Optical Science instrument at the Linac Coherent Light Source as part of the SPI initiative. Existing methods were modified to cope with the shortcomings of the experimental data: inaccessibility of information from half of the detector and a small fraction of single hits. The general SPI analysis workflow was upgraded with the expectation-maximization based classification of diffraction patterns and mode decomposition on the final virus-structure determination step. The presented processing pipeline allowed us to determine the 3D structure of bacteriophage PR772 without symmetry constraints with a spatial resolution of 6.9 nm. The obtained resolution was limited by the scattering intensity during the experiment and the relatively small number of single hits.

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Contributing Institute(s):

1. FS-Photon Science (FS-PS)
2. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
3. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. HRSF-0002 - New avenues in information and data science: advanced imaging applications at the XFEL and cryo-EM frontier (2018_HRSF-0002) (2018_HRSF-0002)
3. AXSIS - Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (609920) (609920)

Experiment(s):

1. Measurement at external facility

Appears in the scientific report 2020

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Database coverage:
; ; ; ; Article Processing Charges ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article (Erratum/Correction) Assalauova, D.XFEL.EU*DESY* ; Kim, Y. Y.XFEL.EU*DESY* ; Bobkov, S. ; Khubbutdinov, R. ; Rose, M.Extern*XFEL.EU* ; Alvarez, R. ; Andreasson, J. ; Balaur, E. ; Contreras, A. ; DeMirci, H. ; Gelisio, L. ; Hajdu, J. ; Hunter, M. S. ; Kurta, R. P. ; Li, H. ; McFadden, M. ; Nazari, R. ; Schwander, P. ; Teslyuk, A. ; Walter, P. ; Xavier, P. L. ; Yoon, C. H. ; Zaare, S. ; Ilyin, V. A. ; Kirian, R. A. ; Hogue, B. G. ; Aquila, A. (Corresponding author) ; Vartanyants, I. A.XFEL.EU*DESY*
Corrigendum: An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser
IUCrJ 9(2), 328 (2022) [10.1107/S2052252522000501]2022     Files BibTeX | EndNote: XML, Text | RIS

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