Home > Publications database > Segmented flow generator for serial crystallography at the European X-ray free electron laser
 
Journal Article PUBDB-2020-03402
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Segmented flow generator for serial crystallography at the European X-ray free electron laser

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2020
Nature Publishing Group UK [London]

Nature Communications 11(1), 4511 () [10.1038/s41467-020-18156-7]
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Abstract: Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.

Classification:
Contributing Institute(s):
  1. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
  2. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
  3. FS-ML (CFEL-XOM)
  4. FS-DS (CFEL-DRD)
Research Program(s):
  1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
  2. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
  3. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
Experiment(s):
  1. Experiments at XFEL

Appears in the scientific report 2020
Database coverage:
Medline ; Creative Commons Attribution CC BY 4.0 ; DOAJ ; OpenAccess ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Essential Science Indicators ; Fees ; IF >= 10 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record
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The record appears in these collections:
Private Collections > >CFEL > >FS-CFEL > CFEL-XOM
Private Collections > >CFEL > >FS-CFEL > CFEL-DRD
Private Collections > >CFEL > >FS-CFEL > CFEL-I
Private Collections > >DESY > >FS > FS-CFEL-1
Document types > Articles > Journal Article
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 Record created 2020-09-14, last modified 2025-07-16
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