Journal Article

PUBDB-2018-02209

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Rapid Sample Delivery for Megahertz Serial Crystallography at X-ray FELs

Wiedorn, M. O. (Corresponding author)CFEL* ; Awel, S.CFEL*DESY* ; Morgan, A. J. ; Ayyer, K. ; Gevorkov, Y. ; Fleckenstein, H.CFEL*XFEL.EU*DESY* ; Roth, N. ; Adriano, L. ; Bean, R. ; Beyerlein, K. R. ; Chen, J. ; Coe, J. ; Cruz-Mazo, F. ; Ekeberg, T. ; Graceffa, R. ; Heymann, M. ; Horke, D. A.Extern*XFEL.EU* ; Knoška, J. ; Mariani, V. ; Nazari, R. ; Oberthür, D. ; Samanta, A. K. ; Sierra, R. G. ; Stan, C. A. ; Yefanov, O. ; Rompotis, D. ; Correa, J. ; Erk, B.XFEL.EU*DESY* ; Treusch, R.DESY* ; Schulz, J. ; Hogue, B. G. ; Gañán-Calvo, A. M. ; Fromme, P.CFEL*Extern* ; Küpper, J.CFEL*XFEL.EU*DESY* ; Rode, A. V. ; Bajt, S.CFEL*XFEL.EU*DESY* ; Kirian, R. A. ; Chapman, H. N.CFEL*XFEL.EU*DESY*

2018
Chester

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Please use a persistent id in citations: doi:10.1107/S2052252518008369  doi:10.3204/PUBDB-2018-02209

Abstract: Liquid microjets are a common means of delivering protein crystals to the focus of X-ray free-electron lasers (FELs) for serial femtosecond crystallography measurements. The high X-ray intensity in the focus initiates an explosion of the microjet and sample. With the advent of X-ray FELs with megahertz rates, the typical velocities of these jets must be increased significantly in order to replenish the damaged material in time for the subsequent measurement with the next X-ray pulse. This work reports the results of a megahertz serial diffraction experiment at the FLASH FEL facility using 4.3 nm radiation. The operation of gas-dynamic nozzles that produce liquid microjets with velocities greater than 80 m s$^{−1}$ was demonstrated. Furthermore, this article provides optical images of X-ray-induced explosions together with Bragg diffraction from protein microcrystals exposed to trains of X-ray pulses repeating at rates of up to 4.5 MHz. The results indicate the feasibility for megahertz serial crystallography measurements with hard X-rays and give guidance for the design of such experiments.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. CFEL-CMI (FS-CFEL-CMI)
3. Uni Hamburg / Experimentalphysik (UNI/EXP)
4. beauftragt von UNI (UNI/CUI)
5. FLASH Wissenschaftlicher Nutzerbetrieb (FS-FLASH-O)
6. Helmholtz-Zentrum Hereon (Hereon)

Research Program(s):

1. 6211 - Extreme States of Matter: From Cold Ions to Hot Plasmas (POF3-621) (POF3-621)
2. 6G2 - FLASH (POF3-622) (POF3-622)
3. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
4. COMOTION - Controlling the Motion of Complex Molecules and Particles (614507) (614507)
5. IVF - Impuls- und Vernetzungsfonds (IVF-20140101) (IVF-20140101)

Experiment(s):

1. FLASH Beamline BL2 (FLASH)
2. FLASH Beamline BL1 (FLASH)
3. PETRA Beamline P05 (PETRA III)

Appears in the scientific report 2018

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Database coverage:
; ; ; ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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