Journal Article

PUBDB-2016-04949

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Serial femtosecond crystallography datasets from G protein-coupled receptors

White, T. (First author)CFEL*DESY* ; Barty, A.CFEL*DESY* ; Liu, W. ; Ishchenko, A. ; Zhang, H. ; Gati, C.CFEL*EMBL* ; Zatsepin, N. A. ; Basu, S. ; Oberthür, D.CFEL*DESY* ; Metz, M.CFEL*DESY* ; Beyerlein, K.CFEL*DESY* ; Yoon, C. H. ; Yefanov, O.CFEL*DESY* ; James, D. ; Wang, D. ; Messerschmidt, M. ; Koglin, J. E. ; Boutet, S. ; Weierstall, U. ; Cherezov, V. (Corresponding author)

2016
Nature Publ. Group London

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Please use a persistent id in citations: doi:10.1038/sdata.2016.57  doi:10.3204/PUBDB-2016-04949

Abstract: We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and delivered in lipidic cubic phase, at the Linac Coherent Light Source. The receptors are: the human serotonin receptor 2B in complex with an agonist ergotamine, the human δ-opioid receptor in complex with a bi-functional peptide ligand DIPP-NH2 , the human smoothened receptor in complex with an antagonist cyclopamine, and finally the human angiotensin II type 1 receptor in complex with the selective antagonist ZD7155. All four datasets have been deposited, with minimal processing, in an HDF5-based file format, which can be used directly for crystallographic processing with CrystFEL or other software. We have provided processing scripts and supporting files for recent versions of CrystFEL, which can be used to validate the data.

Note: URL of special issue: http://www.nature.com/sdata/collections/xfel-biodata

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. 05E13GU1 - Entwicklung eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05E13GU1) (BMBF-05E13GU1)

Experiment(s):

1. Measurement at external facility

Appears in the scientific report 2016

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Database coverage:
; ; ; ; DOAJ Seal ; NCBI Molecular Biology Database

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