Journal Article

PUBDB-2015-04290

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Time-resolved structural studies with serial crystallography: A new light on retinal proteins

Panneels, V. ; Wu, W. ; Tsai, C.-J. ; Nogly, P. ; Rheinberger, J. ; Jaeger, K. ; Cicchetti, G. ; Gati, C.CFEL*>Extern*EMBL*DESY* ; Kick, L. M. ; Sala, L. ; Capitani, G. ; Milne, C. ; Padeste, C. ; Pedrini, B. ; Li, X.-D. ; Standfuss, J. ; Abela, R. ; Schertler, G. (Corresponding author)

2015
AIP Publishing LLC Melville, NY

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Please use a persistent id in citations: doi:10.1063/1.4922774  doi:10.3204/PUBDB-2015-04290

Abstract: Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy. However, these methods do not allow for the structure determination of most intermediate conformations. Recently, the potential of X-Ray Free Electron Lasers (X-FELs) for tracking the dynamics of light-triggered processes by pump-probe serial femtosecond crystallography has been demonstrated using 3D-micron-sized crystals. In addition, X-FELs provide new opportunities for protein 2D-crystal diffraction, which would allow to observe the course of conformational changes of membrane proteins in a close-to-physiological lipid bilayer environment. Here, we describe the strategies towards structural dynamic studies of retinal proteins at room temperature, using injector or fixed-target based serial femtosecond crystallography at X-FELs. Thanks to recent progress especially in sample delivery methods, serial crystallography is now also feasible at synchrotron X-ray sources, thus expanding the possibilities for time-resolved structure determination.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. NANOMEM - Membrane Protein Nanocrystallography (317079) (317079)
3. X-probe - Advanced XFEL and Synchrotron based Probes of Protein Structure and Dynamics (637295) (637295)
4. VH-GS-500 - PIER Helmholtz Graduate School (2015_IFV-VH-GS-500) (2015_IFV-VH-GS-500)

Experiment(s):

1. Experiments at CFEL

Appears in the scientific report 2015

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Database coverage:
; ; SCOPUS

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