Journal Article

PUBDB-2015-04280

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Serial femtosecond crystallography of soluble proteins in lipidic cubic phase

Fromme, R.>Extern* ; Ishchenko, A. ; Metz, M.CFEL* ; Chowdhury, S. R. ; Basu, S.CFEL*DESY* ; Boutet, S.>Extern* ; Fromme, P.>Extern* ; White, T.>Extern* ; Barty, A.CFEL*DESY* ; Spence, J. C. H. ; Weierstall, U.>Extern* ; Liu, W.>Extern* ; Cherezov, V. (Corresponding author)

2015
International Union of Crystallography (IUCr) Chester

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Please use a persistent id in citations: doi:10.1107/S2052252515013160  doi:10.3204/PUBDB-2015-04280

Abstract: Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP–SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubicphase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. NANOMEM - Membrane Protein Nanocrystallography (317079) (317079)
3. CUI - Hamburger Zentrum für ultraschnelle Beobachtung (194651731) (194651731)

Experiment(s):

1. Experiments at CFEL
2. Measurement at external facility

Appears in the scientific report 2015

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Database coverage:
; ; ; ; Current Contents - Physical, Chemical and Earth Sciences ; NCBI Molecular Biology Database ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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