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000275863 1001_ $$0P:(DE-H253)PIP1026130$$aFromme, Raimund$$b0
000275863 245__ $$aSerial femtosecond crystallography of soluble proteins in lipidic cubic phase
000275863 260__ $$aChester$$bInternational Union of Crystallography (IUCr)$$c2015
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000275863 520__ $$aSerial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations.  An  LCP–SFX  method  has  recently  been  introduced  in  which microcrystals  of  membrane  proteins  are  grown  and  delivered  for  SFX  data collection  inside  a  gel-like  membrane-mimetic  matrix,  known  as  lipidic  cubicphase   (LCP),   using   a   special   LCP   microextrusion   injector.   Here,   it   is demonstrated  that  LCP  can  also  be  used  as  a  suitable  carrier  medium  for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of  crystallized  protein  required  for  data  collection  compared  with  crystals delivered by liquid injectors. High-quality LCP–SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
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000275863 536__ $$0G:(EU-Grant)317079$$aNANOMEM - Membrane Protein Nanocrystallography (317079)$$c317079$$fFP7-PEOPLE-2012-ITN$$x1
000275863 536__ $$0G:(GEPRIS)194651731$$aCUI - Hamburger Zentrum für ultraschnelle Beobachtung (194651731)$$c194651731$$x2
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000275863 7001_ $$0P:(DE-HGF)0$$aIshchenko, Andrii$$b1
000275863 7001_ $$0P:(DE-H253)PIP1019892$$aMetz, Markus$$b2
000275863 7001_ $$0P:(DE-HGF)0$$aChowdhury, Shatabdi Roy$$b3
000275863 7001_ $$0P:(DE-H253)PIP1016420$$aBasu, Shibom$$b4$$udesy
000275863 7001_ $$0P:(DE-H253)PIP1009086$$aBoutet, Sébastien$$b5
000275863 7001_ $$0P:(DE-H253)PIP1023170$$aFromme, Petra$$b6
000275863 7001_ $$0P:(DE-H253)PIP1010779$$aWhite, Thomas$$b7
000275863 7001_ $$0P:(DE-H253)PIP1008245$$aBarty, Anton$$b8$$udesy
000275863 7001_ $$0P:(DE-HGF)0$$aSpence, John C. H.$$b9
000275863 7001_ $$0P:(DE-H253)PIP1008265$$aWeierstall, Uwe$$b10
000275863 7001_ $$0P:(DE-H253)PIP1021486$$aLiu, Wei$$b11
000275863 7001_ $$0P:(DE-HGF)0$$aCherezov, Vadim$$b12$$eCorresponding author
000275863 773__ $$0PERI:(DE-600)2754953-7$$a10.1107/S2052252515013160$$gVol. 2, no. 5, p. 545 - 551$$n5$$p545 - 551$$tIUCrJ$$v2$$x2052-2525$$y2015
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000275863 9141_ $$y2015
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