Journal Article

PUBDB-2015-00661

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Calponin-Like Chd64 Is Partly Disordered

Kozlowska , M.>Extern* ; Tarczewska, A.>Extern* ; michał, j. ; Szpotkowski, K.>Extern* ; Wojtas, M.>Extern* ; Andrzej, O. (Corresponding author)

2014
PLoS Lawrence, Kan.

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Please use a persistent id in citations: doi:10.1371/journal.pone.0096809

Abstract: 20-hydroxyecdysone (20E) and juvenile hormone (JH) signaling pathways interact to regulate insect development. Recently,two proteins, a calponin-like Chd64 and immunophilin FKBP39 have been found to play a pivotal role in the cross-talkbetween 20E and JH, although the molecular basis of interaction remains unknown. The aim of this work was to identify thestructural features that would provide understanding of the role of Chd64 in multiple and dynamic complex that cross-linksthe signaling pathways. Here, we demonstrate the results of in silico and in vitro analyses of the structural organization ofChd64 from Drosophila melanogaster and its homologue from Tribolium castaneum. Computational analysis predicted theexistence of disordered regions on the termini of both proteins, while the central region appeared to be globular, probablycorresponding to the calponin homology (CH) domain. In vitro analyses of the hydrodynamic properties of the proteinsfrom analytical size-exclusion chromatography and analytical ultracentrifugation revealed that DmChd64 and TcChd64 hadan asymmetrical, elongated shape, which was further confirmed by small angle X-ray scattering (SAXS). The Kratky plotindicated disorderness in both Chd64 proteins, which could possibly be on the protein termini and which would give rise tospecific hydrodynamic properties. Disordered tails are often involved in diverse interactions. Therefore, it is highly possiblethat there are intrinsically disordered regions (IDRs) on both termini of the Chd64 proteins that serve as platforms formultiple interaction with various partners and constitute the foundation for their regulatory function.

Note: OA

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Contributing Institute(s):

1. EMBL-User (EMBL-User)

Research Program(s):

1. PETRA Beamline P13 (POF2-54G14) (POF2-54G14)

Experiment(s):

1. PETRA Beamline P13 (PETRA III)

Appears in the scientific report 2014

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Database coverage:
; ; ; ; BIOSIS Previews ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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