TY  - JOUR
AU  - Kozlowska , Malgorzata
AU  - Tarczewska, Aneta
AU  - michał, jakób
AU  - Szpotkowski, Kamil
AU  - Wojtas, Magdalena
AU  - Andrzej, Ozyhar
TI  - Calponin-Like Chd64 Is Partly Disordered
JO  - PLoS one
VL  - 9
IS  - 5
SN  - 1932-6203
CY  - Lawrence, Kan.
PB  - PLoS
M1  - PUBDB-2015-00661
SP  - e96809
PY  - 2014
N1  - OA
AB  - 20-hydroxyecdysone (20E) and juvenile hormone (JH) signaling pathways interact to regulate insect development. Recently,two proteins, a calponin-like Chd64 and immunophilin FKBP39 have been found to play a pivotal role in the cross-talkbetween 20E and JH, although the molecular basis of interaction remains unknown. The aim of this work was to identify thestructural features that would provide understanding of the role of Chd64 in multiple and dynamic complex that cross-linksthe signaling pathways. Here, we demonstrate the results of in silico and in vitro analyses of the structural organization ofChd64 from Drosophila melanogaster and its homologue from Tribolium castaneum. Computational analysis predicted theexistence of disordered regions on the termini of both proteins, while the central region appeared to be globular, probablycorresponding to the calponin homology (CH) domain. In vitro analyses of the hydrodynamic properties of the proteinsfrom analytical size-exclusion chromatography and analytical ultracentrifugation revealed that DmChd64 and TcChd64 hadan asymmetrical, elongated shape, which was further confirmed by small angle X-ray scattering (SAXS). The Kratky plotindicated disorderness in both Chd64 proteins, which could possibly be on the protein termini and which would give rise tospecific hydrodynamic properties. Disordered tails are often involved in diverse interactions. Therefore, it is highly possiblethat there are intrinsically disordered regions (IDRs) on both termini of the Chd64 proteins that serve as platforms formultiple interaction with various partners and constitute the foundation for their regulatory function.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000335728900099
C6  - pmid:24805353
DO  - DOI:10.1371/journal.pone.0096809
UR  - https://bib-pubdb1.desy.de/record/206135
ER  -