Journal Article

DESY-2014-02888

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Diffraction before destruction

Chapman, H. N. (Corresponding Author)DESY*>CFEL* ; Caleman, C.DESY*>CFEL* ; Timneanu, N.Extern*

2014
JSTOR London

This record in other databases:        

Please use a persistent id in citations: doi:10.1098/rstb.2013.0313

Abstract: X-ray free-electron lasers have opened up the possibility of structure determination of protein crystals at room temperature, free of radiation damage. The femtosecond-duration pulses of these sources enable diffraction signals to becollected from samples at doses of 1000 MGy or higher. The sample is vaporized by the intense pulse, but not before the scattering that gives rise to the diffraction pattern takes place. Consequently, only a single flash diffraction pattern can be recorded from a crystal, giving rise to the method of serial crystallography where tens of thousands of patterns are ollected from individual crystals that flow across the beam and the patterns are indexed and aggregated into a set of structure factors. The high-dose tolerance and the many-crystal averaging approach allow data to be collected from much smaller crystals than have been examined at synchrotron radiation facilities, even from radiation-sensitive samples. Here, we review the interaction of intense femtosecond X-ray pulses with materials and discuss the implications forstructure determination. We identify various dose regimes and conclude that the strongest achievable signals for a given sample are attained at the highest possible dose rates, from highest possible pulse intensities.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. Experiments at CFEL (POF2-544) (POF2-544)

Experiment(s):

1. Experiments at CFEL

Appears in the scientific report 2014

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Database coverage:
; ; ; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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