Journal Article

DESY-2014-02800

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Weierstall, U. (Corresponding Author)Extern* ; James, D. ; Wang, C. ; White, T.DESY*>CFEL* ; Wang, D. ; Liu, W.Extern* ; Spence, J. C. H. ; Bruce Doak, R. ; Nelson, G. ; Fromme, P. ; Fromme, R. ; Grotjohann, I. ; Kupitz, C. ; Zatsepin, N. A. ; Liu, H. ; Basu, S. ; Wacker, D. ; Won Han, G. ; Katritch, V. ; Boutet, S.Extern* ; Messerschmidt, M.Extern* ; Williams, G. J. ; Koglin, J. E. ; Marvin Seibert, M. ; Klinker, M. ; Gati, C.Extern*>CFEL* ; Shoeman, R. L. ; Barty, A.DESY*>CFEL* ; Chapman, H. N.DESY*>CFEL* ; Kirian, R.DESY*>CFEL* ; Beyerlein, K.DESY*>CFEL* ; Stevens, R. C. ; Li, D. ; Shah, S. T. A. ; Howe, N. ; Caffrey, M. ; Cherezov, V.

2014
Macmillan Publishers Limited London

This record in other databases:      

Please use a persistent id in citations: doi:10.1038/ncomms4309

Abstract: Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. Experiments at CFEL (POF2-544) (POF2-544)

Experiment(s):

1. Experiments at CFEL

Appears in the scientific report 2014

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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